Bacterial flora and antibiotic resistance from eggs of green turtles Chelonia mydas

An indication of polluted effluents

Saif Al-Bahry, Ibrahim Mahmoud, Abdulkader Elshafie, Asila Al-Harthy, Sabha Al-Ghafri, Issa Al-Amri, Abdulaziz Alkindi

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Sea turtles migrate to various habitats where they can be exposed to different pollutants. Bacteria were collected from turtle eggs and their resistance to antibiotics was used as pollutant bio-indicators of contaminated effluents. Eggs were collected randomly from turtles when they were laying their eggs. A total of 90 eggs were collected and placed into sterile plastic bags (3 eggs/turtle) during June-December of 2003. The bacteria located in the eggshell, albumen and yolk were examined, and 42% of the eggs were contaminated with 10 genera of bacteria. Pseudomonas spp. were the most frequent isolates. The albumen was found to be the part of the egg to be the least contaminated by bacterial infection. Bacterial isolates tested with 14 antibiotics showed variations in resistance. Resistance to ampicillin was the highest. The presence of antibiotic resistant bacteria in eggs indicates that the green turtle populations were subjected to polluted effluents during some of their migratory routes and feeding habitats. Scanning electron microscopy revealed that Salmonella typhimurium penetrated all eggshell layers.

Original languageEnglish
Pages (from-to)720-725
Number of pages6
JournalMarine Pollution Bulletin
Volume58
Issue number5
DOIs
Publication statusPublished - May 2009

Fingerprint

Chelonia mydas
antibiotic resistance
Antibiotics
turtle
effluents
Effluents
Bacteria
flora
turtles
effluent
egg
antibiotics
bacteria
egg shell
pollutants
eggshell
bacterium
Salmonella
plastic bags
sea turtles

Keywords

  • Antibiotics
  • Bacteria
  • Eggs
  • Green turtles
  • Pollution

ASJC Scopus subject areas

  • Aquatic Science
  • Oceanography
  • Pollution

Cite this

Bacterial flora and antibiotic resistance from eggs of green turtles Chelonia mydas : An indication of polluted effluents. / Al-Bahry, Saif; Mahmoud, Ibrahim; Elshafie, Abdulkader; Al-Harthy, Asila; Al-Ghafri, Sabha; Al-Amri, Issa; Alkindi, Abdulaziz.

In: Marine Pollution Bulletin, Vol. 58, No. 5, 05.2009, p. 720-725.

Research output: Contribution to journalArticle

Al-Bahry, Saif ; Mahmoud, Ibrahim ; Elshafie, Abdulkader ; Al-Harthy, Asila ; Al-Ghafri, Sabha ; Al-Amri, Issa ; Alkindi, Abdulaziz. / Bacterial flora and antibiotic resistance from eggs of green turtles Chelonia mydas : An indication of polluted effluents. In: Marine Pollution Bulletin. 2009 ; Vol. 58, No. 5. pp. 720-725.
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