Bacterial communities in the rhizosphere of Phragmites australis from an oil-polluted wetland

Raeid M.M. Abed, Samiha Al-Kharusi, Panagiotis Gkorezis, Stephane Prigent, Tom Headley

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Although Phragmites australis is commonly planted in constructed wetlands, very little is known about its roots-associated bacterial communities, especially in wetlands used for the remediation of oil produced waters. Here, we describe the bacterial diversity, using molecular (illumina MiSeq sequencing) and cultivation techniques, in the rhizosphere soils of P. australis from an oil-polluted wetland in Oman. The obtained isolates were tested for their plant-growth promoting properties. Most sequences belonged to Proteobacteria, Bacteriodetes and Firmicutes. Sequences of potential hydrocarbon-degrading bacteria (e.g. Ochrobactrum, and Pseudomonas) were frequently encountered. All soils contained sequences of known sulfur-oxidizing (e.g. Thiobacillus, Thiofaba, Rhodobacter and Sulfurovum) and sulfate-reducing bacteria, although the latter group made up only 0.1% to 3% of total sequences. The obtained isolates from the rhizosphere soils were phylogenetically affiliated to Serratia, Acinetobacter, Xenorhabdus, Escherichia and Salmonella. All strains were able to solubilize phosphate and about half were capable of producing organic acids and 1-aminocyclopropane-1-carboxylate (ACC) deaminase. Around 42% of the strains had the ability to produce indole acetic acid and siderophores. We conclude that the rhizosphere soils of P. australis in oil polluted wetlands harbor diverse bacterial communities that could enhance the wetland performance through hydrocarbon degradation, nutrient cycling and supporting plant growth.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalArchives of Agronomy and Soil Science
DOIs
Publication statusAccepted/In press - Jul 20 2017

Fingerprint

Phragmites australis
bacterial communities
rhizosphere
wetlands
wetland
oils
oil
hydrocarbons
Rhodobacter
soil
Ochrobactrum
Thiobacillus
Xenorhabdus
1-aminocyclopropane-1-carboxylate deaminase
plant growth
soil sequences
hydrocarbon
Serratia
Escherichia
sulfate-reducing bacteria

Keywords

  • constructed wetland
  • hydrocarbons
  • MiSeq sequencing
  • P. australis
  • Reed bed

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Soil Science

Cite this

Bacterial communities in the rhizosphere of Phragmites australis from an oil-polluted wetland. / Abed, Raeid M.M.; Al-Kharusi, Samiha; Gkorezis, Panagiotis; Prigent, Stephane; Headley, Tom.

In: Archives of Agronomy and Soil Science, 20.07.2017, p. 1-11.

Research output: Contribution to journalArticle

Abed, Raeid M.M. ; Al-Kharusi, Samiha ; Gkorezis, Panagiotis ; Prigent, Stephane ; Headley, Tom. / Bacterial communities in the rhizosphere of Phragmites australis from an oil-polluted wetland. In: Archives of Agronomy and Soil Science. 2017 ; pp. 1-11.
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