Association of Tomato yellow leaf curl virus and Chili leaf curl virus with leaf curl disease of radish and the synergistic interaction on Nicotiana benthamiana

Adel A. Al-Shihi, e Ammara Um e Ammara, Imran Amin, Michael Deadman, Abdullah M. Al-Sadi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Leaf yellowing and rolling with slight crinkling was observed in radish plants in Oman, indicative of a begomovirus infection. Viral sequences from radish were 99% identical to sequences of Tomato yellow leaf curl virus (TYLCV) and Chilli leaf curl virus (ChiLCV), from Oman. No beta- or alpha-satellites were detected. Infectious clones were developed and infectivity analyses of isolated viruses were done in a model host Nicotiana benthamiana. Both viruses, when inoculated separately, produced typical symptoms; however, symptom severity increased when these viruses were co-inoculated. Southern analyses confirmed that virus accumulation was high when both viruses were inoculated as compared to independent inoculation. Real-time qPCR analyses showed that copy number of both viruses was 1 X 106 copies when N. benthamiana plants were inoculated with either TYLCV or ChiLCV; however, the copy number increased to 1 X 108 copies when the two viruses were co-inoculated together in N. benthamiana.

Original languageEnglish
Pages (from-to)266-272
Number of pages7
JournalInternational Journal of Agriculture and Biology
Volume19
Issue number2
DOIs
Publication statusPublished - 2017

Fingerprint

Tomato yellow leaf curl virus
Raphanus
leaf curling
Nicotiana benthamiana
Lycopersicon esculentum
radishes
Tobacco
Viruses
viruses
Oman
signs and symptoms (plants)
Begomovirus
Chilli leaf curl virus
pathogenicity
clones
Clone Cells
infection

Keywords

  • Begomovirus
  • ChiLCV
  • Mixed infection
  • Radish
  • TYLCV

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Association of Tomato yellow leaf curl virus and Chili leaf curl virus with leaf curl disease of radish and the synergistic interaction on Nicotiana benthamiana. / Al-Shihi, Adel A.; Um e Ammara, e Ammara; Amin, Imran; Deadman, Michael; Al-Sadi, Abdullah M.

In: International Journal of Agriculture and Biology, Vol. 19, No. 2, 2017, p. 266-272.

Research output: Contribution to journalArticle

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