Association of three begomoviruses and a betasatellite with leaf curl disease of basil in Oman

Um E. Ammara, Mahmoud Al-Ansari, Adel Al-Shihi, Imran Amin, Shahid Mansoor, Ahmad Y. Al-Maskari, Abdullah M. Al-Sadi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Basil (Ocimum basilicum) is an important medicinal and aromatic plant native to Asia and Africa. Among 30 different species of Ocimum, common basil is the most widely cultivated species in Oman. During a survey in ASeeb, a wilayat of Muscat, Oman in 2014, disease symptoms such as severe leaf curling, yellowing, and crinkling were observed on basil plants. To confirm the potential association of begomovirus with this disease, symptomatic leaves were collected, nucleic acid was extracted, and rolling circle amplification (RCA) using Phi 29 DNA polymerase was performed. Restriction analysis of the amplified product was conducted and full-length clones of a putative begomovirus and betasatellite were isolated and characterized. Sequence analyses of several clones showed that the basil plants were infected with Tomato yellow leaf curl virus-Oman (TYLCV-OM), Chili leaf curl virus-Oman (ChLCV-OM), and Tomato leaf curl Albatinah virus (ToLCABV). Infected basil plants also harboured Tomato leaf curl betasatellite-Oman (ToLCB-OM), a satellite associated with leaf curl disease of tomato in Oman. All efforts to clone alphasatellites were unsuccessful, indicating that an alphasatellite was not associated with this infection. This is the first report of the association of multiple begomoviruses along with a betasatellite in the leaf curl disease of basil.

Original languageEnglish
Pages (from-to)506-513
Number of pages8
JournalCanadian Journal of Plant Pathology
Volume37
Issue number4
DOIs
Publication statusPublished - Oct 2 2015

Fingerprint

Begomovirus
leaf curling
basil
Oman
clones
Tomato leaf curl virus
tomatoes
Ocimum
Tomato yellow leaf curl virus
Ocimum basilicum
essential oil crops
foliar diseases
DNA-directed DNA polymerase
nucleic acids
signs and symptoms (plants)
medicinal plants
infection

Keywords

  • Begomovirus
  • betasatellite
  • Geminivirus
  • leaf curl disease
  • Ocimum basilicum L.

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Association of three begomoviruses and a betasatellite with leaf curl disease of basil in Oman. / Ammara, Um E.; Al-Ansari, Mahmoud; Al-Shihi, Adel; Amin, Imran; Mansoor, Shahid; Al-Maskari, Ahmad Y.; Al-Sadi, Abdullah M.

In: Canadian Journal of Plant Pathology, Vol. 37, No. 4, 02.10.2015, p. 506-513.

Research output: Contribution to journalArticle

Ammara, Um E. ; Al-Ansari, Mahmoud ; Al-Shihi, Adel ; Amin, Imran ; Mansoor, Shahid ; Al-Maskari, Ahmad Y. ; Al-Sadi, Abdullah M. / Association of three begomoviruses and a betasatellite with leaf curl disease of basil in Oman. In: Canadian Journal of Plant Pathology. 2015 ; Vol. 37, No. 4. pp. 506-513.
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