Association of the methylenetetrahydrofolate reductase A1298C but not the C677T single nucleotide polymorphism with sickle cell disease in Bahrain

Iman K. Al-Absi, Abeer M. Al-Subaie, Ghada Ameen, Najat Mahdi, Akbar Mohsin Mohammad, Naglaa A. Fawaz, Wassim Y. Almawi*

*Corresponding author for this work

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The association of methylenetetrahydrofolate reductase (MTHFR) gene mutations, C677T and A1298C, together with changes in homocysteine (Hcy) levels was investigated in 106 sickle cell disease patients and 156 healthy controls from Bahrain. The mutation analysis was done by restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR). While the frequencies of the mutant alleles C677T and A1298C were comparable between patients and controls, the frequency of the A1298C (C/C) (p = 0.03) but not C677T (T/T) (p = 0.67) genotype, and of the 677T/1298C haplotype were significantly higher in the patients (p = 0.05). Homocysteine levels were normal in all subjects. This suggests that the A1298C, but not C677T, mutation is associated with the genotype of sickle cell disease.

Original languageEnglish
Pages (from-to)449-453
Number of pages5
JournalHemoglobin
Volume30
Issue number4
DOIs
Publication statusPublished - Dec 1 2006

Keywords

  • Homocysteine (Hcy)
  • Methylenetetrahydrofolate reductase (MTHFR)
  • Mutation
  • Restriction fragment length polymorphism-polymerase chain reaction (RFLP-PCR)
  • Sickle cell disease

ASJC Scopus subject areas

  • Hematology
  • Clinical Biochemistry
  • Genetics(clinical)
  • Biochemistry, medical

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