Aromatic-dependent Salmonella dublin as a parenteral modified live vaccine for calves.

B. P. Smith, M. Reina-Guerra, B. A. Stocker, S. K. Hoiseth, E. Johnson

Research output: Contribution to journalArticle

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Abstract

A virulent Salmonella dublin isolate was made histidine-requiring (his-) to allow recognition. The his- derivative, SL1367 (still calf-virulent), was then given by transduction and mutation, a transposon-generated non-reverting aromatic biosynthesis (aro) defect; this defect caused loss of virulence for the mouse. The his- aro- derivative strain, SL1438, was effective as a live vaccine in mice. Twenty male Holstein calves were divided into 4 groups. Groups I, II, and III were vaccinated IM at 2 weeks and at 3 weeks of age with aromatic-dependent (aro-) S dublin strain SL1438. Groups I and III received freshly prepared vaccine and group II received lyophilized vaccine. Serious adverse reactions to the vaccination were not seen. After vaccination, the mean maximum increase in rectal temperature was 1.8 C in group I and III calves and 0.6 C in group II calves. Fewer group II calves developed diarrhea (1 of 5) or positive blood cultures (0 of 5) after vaccination compared with group I and III calves (6 of 10 and 5 of 10, respectively). Postvaccination diarrhea was mild and of short duration. Group IV was comprised of 5 nonvaccinated calves. At 5 weeks of age, all calves were challenge exposed orally. Group I, II, and IV calves were challenge exposed with 10(11) virulent S dublin SL1367. Group III was challenge exposed with 10(11) virulent S typhimurium UCD 108-11. Subsequently, fever and diarrhea (lasting 1 to 3 days), but no deaths, were observed in the vaccinated calves. Four of the 5 nonvaccinated (group IV) calves died (P less than 0.001) within 8 days after challenge exposure. Aro- S dublin SL 1438 did not cause serious adverse effects and provided protection against oral challenge exposure with either virulent S dublin or S typhimurium.

Original languageEnglish
Pages (from-to)2231-2235
Number of pages5
JournalAmerican Journal of Veterinary Research
Volume45
Issue number11
Publication statusPublished - Nov 1984

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Salmonella Dublin
live vaccines
Histidine
Salmonella
aromatic compounds
Diarrhea
Vaccination
Vaccines
calves
Virulence
histidine
Fever
diarrhea
Mutation
vaccination
Temperature
vaccines
mice
transposons
fever

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Aromatic-dependent Salmonella dublin as a parenteral modified live vaccine for calves. / Smith, B. P.; Reina-Guerra, M.; Stocker, B. A.; Hoiseth, S. K.; Johnson, E.

In: American Journal of Veterinary Research, Vol. 45, No. 11, 11.1984, p. 2231-2235.

Research output: Contribution to journalArticle

Smith, BP, Reina-Guerra, M, Stocker, BA, Hoiseth, SK & Johnson, E 1984, 'Aromatic-dependent Salmonella dublin as a parenteral modified live vaccine for calves.', American Journal of Veterinary Research, vol. 45, no. 11, pp. 2231-2235.
Smith, B. P. ; Reina-Guerra, M. ; Stocker, B. A. ; Hoiseth, S. K. ; Johnson, E. / Aromatic-dependent Salmonella dublin as a parenteral modified live vaccine for calves. In: American Journal of Veterinary Research. 1984 ; Vol. 45, No. 11. pp. 2231-2235.
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