Antimicrobial peptides from the skin secretions of the New World frogs Lithobates capito and Lithobates warszewitschii (Ranidae)

J. Michael Conlon, Mohammed A. Meetani, Laurent Coquet, Thierry Jouenne, Jérôme Leprince, Hubert Vaudry, Jolanta Kolodziejek, Norbert Nowotny, Jay D. King

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Taxonomic revisions within the anuran family Ranidae have established the genus Lithobates that currently comprises 49 species of frogs from the New World. Peptidomic analysis, using reversed-phase HPLC with on-line detection by electrospray mass spectrometry, has led to the identification of multiple antimicrobial peptides in norepinephrine-stimulated skin secretions of the North American frog Lithobates capito and the Central American frog Lithobates warszewitschii. Structural characterization of the peptides demonstrated that the L. capito secretions contained brevinin-1 (1), esculentin-1 (1), esculentin-2 (1), ranatuerin-2 (3), and temporin (2) peptides. L. warszewitschii secretions contained brevinin-1 (1), esculentin-2 (1), ranatuerin-2 (2), and temporin (1) peptides. Values in parentheses indicate number of peptides in each family. Temporin-CPa from L. capito, with the atypical structure IPPFIKKVLTTVF·NH2, also showed atypical growth-inhibitory activity having greater potency against Escherichia coli (MIC = 25 μM) and Candida albicans (MIC = 25 μM) than against Staphylococcus aureus (MIC = 50 μM). Phylogenetic analysis based upon the amino acid sequences of 37 ranatuerin-2 peptides from 17 species belonging to the genus Lithobates provides support for currently accepted taxonomic relationships. L. capito is sister-group to Lithobates sevosus in a clade that also contains Lithobates areolatus, and Lithobates palustris. L. warszewitschii is most closely related to the Central American species Lithobates tarahumarae and Lithobates vaillanti.

Original languageEnglish
Pages (from-to)1775-1781
Number of pages7
JournalPeptides
Volume30
Issue number10
DOIs
Publication statusPublished - Oct 2009

Fingerprint

Ranidae
Anura
Skin
Peptides
Candida
Candida albicans
Escherichia coli
Mass spectrometry
Staphylococcus aureus
Amino Acid Sequence
Mass Spectrometry
Norepinephrine
High Pressure Liquid Chromatography
Amino Acids
Growth
temporin
esculentin steroid
ranatuerin

Keywords

  • Antimicrobial peptide
  • Frog skin
  • Lithobates
  • Ranatuerin-2
  • Temporin

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Conlon, J. M., Meetani, M. A., Coquet, L., Jouenne, T., Leprince, J., Vaudry, H., ... King, J. D. (2009). Antimicrobial peptides from the skin secretions of the New World frogs Lithobates capito and Lithobates warszewitschii (Ranidae). Peptides, 30(10), 1775-1781. https://doi.org/10.1016/j.peptides.2009.07.011

Antimicrobial peptides from the skin secretions of the New World frogs Lithobates capito and Lithobates warszewitschii (Ranidae). / Conlon, J. Michael; Meetani, Mohammed A.; Coquet, Laurent; Jouenne, Thierry; Leprince, Jérôme; Vaudry, Hubert; Kolodziejek, Jolanta; Nowotny, Norbert; King, Jay D.

In: Peptides, Vol. 30, No. 10, 10.2009, p. 1775-1781.

Research output: Contribution to journalArticle

Conlon, JM, Meetani, MA, Coquet, L, Jouenne, T, Leprince, J, Vaudry, H, Kolodziejek, J, Nowotny, N & King, JD 2009, 'Antimicrobial peptides from the skin secretions of the New World frogs Lithobates capito and Lithobates warszewitschii (Ranidae)', Peptides, vol. 30, no. 10, pp. 1775-1781. https://doi.org/10.1016/j.peptides.2009.07.011
Conlon, J. Michael ; Meetani, Mohammed A. ; Coquet, Laurent ; Jouenne, Thierry ; Leprince, Jérôme ; Vaudry, Hubert ; Kolodziejek, Jolanta ; Nowotny, Norbert ; King, Jay D. / Antimicrobial peptides from the skin secretions of the New World frogs Lithobates capito and Lithobates warszewitschii (Ranidae). In: Peptides. 2009 ; Vol. 30, No. 10. pp. 1775-1781.
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