Antimicrobial peptides from ranid frogs: Taxonomic and phylogenetic markers and a potential source of new therapeutic agents

J. Michael Conlon, Jolanta Kolodziejek, Norbert Nowotny

Research output: Contribution to journalArticle

316 Citations (Scopus)

Abstract

Granular glands in the skins of frogs of the genus Rana, a widely distributed group with over 250 species, synthesize and secrete a remarkably diverse array of peptides with antimicrobial activity that are believed to have arisen as a result of multiple gene duplication events. Almost without exception, these components are hydrophobic, cationic and form an amphipathic α-helix in a membrane-mimetic solvent. The peptides can be grouped into families on the basis of structural similarity. To date, brevinin-1, esculentin-1, esculentin-2, and temporin peptides have been found in ranid frogs of both Eurasian and North American origin; ranalexin, ranatuerin-1, ranatuerin-2 and palustrin peptides only in N. American frogs; and brevinin-2, tigerinin, japonicin, nigrocin and melittin-related peptides only in Eurasian frogs. It is generally assumed that this structurally diversity serves to protect the organism against a wide range of pathogens but convincing evidence in support of this hypothesis is still required. The possibility that "antimicrobial peptides" fulfill additional or alternative biological functions should not be rejected. The molecular heterogeneity of the peptide families, particularly brevinin-1, brevinin-2 and ranatuerin-2, may be exploited for the purposes of unequivocal identification of specimens and for an understanding of phylogenetic interrelationships between species. The broad-spectrum antibacterial and antifungal activities of certain peptides, for example esculentin-1, ranalexin-1 and ranatuerin, together with their relatively low hemolytic activity, make them candidates for development into therapeutically useful anti-infective agents.

Original languageEnglish
Pages (from-to)1-14
Number of pages14
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1696
Issue number1
DOIs
Publication statusPublished - Jan 14 2004

Fingerprint

Anura
Peptides
Therapeutics
Melitten
Ranidae
Gene Duplication
Pathogens
Anti-Infective Agents
Skin
Genes
Membranes
ranatuerin
esculentin steroid

Keywords

  • Antimicrobial peptide
  • Drug development
  • Phylogeny
  • Rana
  • Taxonomy

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics

Cite this

Antimicrobial peptides from ranid frogs : Taxonomic and phylogenetic markers and a potential source of new therapeutic agents. / Conlon, J. Michael; Kolodziejek, Jolanta; Nowotny, Norbert.

In: Biochimica et Biophysica Acta - Proteins and Proteomics, Vol. 1696, No. 1, 14.01.2004, p. 1-14.

Research output: Contribution to journalArticle

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