Abstract
Dendrophthoe falcata is a hemiparasitic plant commonly used for ailments such as ulcers, asthma, impotence, paralysis, skin diseases, menstrual troubles, pulmonary tuberculosis, and wounds. In this context, the validations of the traditional claim that the leaf extract of D. falcata possesses antibiofilm and anti-quorum sensing activity against different bacterial pathogens were assessed. The bacterial biofilms were quantified by crystal violet staining. Among the 17 bacterial pathogens screened, the methanolic fraction of the leaf extract clearly demonstrated antibiofilm activity for Proteus mirabilis, Vibrio vulnificus, Aeromonas hydrophila, Shigella sonnei, Chromobacterium violaceum ATCC 12472, Vibrio parahaemolyticus, Vibrio harveyi, Vibrio alginolyticus, Vibrio cholerae, and Proteus vulgaris. At biofilm inhibitory concentrations, biofilm formation was reduced by up to 70-90%. Furthermore, the potential quorum-sensing activity of the leaf extract was tested by agar well diffusion using Chromobacterium violaceum (ATCC 12472 & CV O26) reporter strains. The inhibition of violacein production may be due to direct or indirect interference on QS by active constituents or the interactive effect of different phytocompounds present in the extracts. This is the first report on antibiofilm and QS activity of D. falcata leaf extracts, signifying the scope for development of complementary medicine for biofilm-associated infections.
| Original language | English |
|---|---|
| Pages (from-to) | 1918-1926 |
| Number of pages | 9 |
| Journal | Planta Medica |
| Volume | 78 |
| Issue number | 18 |
| DOIs | |
| Publication status | Published - 2012 |
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Keywords
- bacterial pathogens
- biofilm
- confocal laser scanning microscopy
- Dendrophthoe falcata
- Loranthaceae
- quorum sensing
ASJC Scopus subject areas
- Complementary and alternative medicine
- Molecular Medicine
- Organic Chemistry
- Analytical Chemistry
- Pharmaceutical Science
- Pharmacology
- Drug Discovery
Cite this
Antibiofilm activity of dendrophthoe falcata against different bacterial pathogens. / Karthikeyan, Alagarsamy; Rameshkumar, Ramakrishnan; Sivakumar, Nallusamy; Al Amri, Issa S.; Karutha Pandian, Shunmugiah; Ramesh, Manikandan.
In: Planta Medica, Vol. 78, No. 18, 2012, p. 1918-1926.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Antibiofilm activity of dendrophthoe falcata against different bacterial pathogens
AU - Karthikeyan, Alagarsamy
AU - Rameshkumar, Ramakrishnan
AU - Sivakumar, Nallusamy
AU - Al Amri, Issa S.
AU - Karutha Pandian, Shunmugiah
AU - Ramesh, Manikandan
PY - 2012
Y1 - 2012
N2 - Dendrophthoe falcata is a hemiparasitic plant commonly used for ailments such as ulcers, asthma, impotence, paralysis, skin diseases, menstrual troubles, pulmonary tuberculosis, and wounds. In this context, the validations of the traditional claim that the leaf extract of D. falcata possesses antibiofilm and anti-quorum sensing activity against different bacterial pathogens were assessed. The bacterial biofilms were quantified by crystal violet staining. Among the 17 bacterial pathogens screened, the methanolic fraction of the leaf extract clearly demonstrated antibiofilm activity for Proteus mirabilis, Vibrio vulnificus, Aeromonas hydrophila, Shigella sonnei, Chromobacterium violaceum ATCC 12472, Vibrio parahaemolyticus, Vibrio harveyi, Vibrio alginolyticus, Vibrio cholerae, and Proteus vulgaris. At biofilm inhibitory concentrations, biofilm formation was reduced by up to 70-90%. Furthermore, the potential quorum-sensing activity of the leaf extract was tested by agar well diffusion using Chromobacterium violaceum (ATCC 12472 & CV O26) reporter strains. The inhibition of violacein production may be due to direct or indirect interference on QS by active constituents or the interactive effect of different phytocompounds present in the extracts. This is the first report on antibiofilm and QS activity of D. falcata leaf extracts, signifying the scope for development of complementary medicine for biofilm-associated infections.
AB - Dendrophthoe falcata is a hemiparasitic plant commonly used for ailments such as ulcers, asthma, impotence, paralysis, skin diseases, menstrual troubles, pulmonary tuberculosis, and wounds. In this context, the validations of the traditional claim that the leaf extract of D. falcata possesses antibiofilm and anti-quorum sensing activity against different bacterial pathogens were assessed. The bacterial biofilms were quantified by crystal violet staining. Among the 17 bacterial pathogens screened, the methanolic fraction of the leaf extract clearly demonstrated antibiofilm activity for Proteus mirabilis, Vibrio vulnificus, Aeromonas hydrophila, Shigella sonnei, Chromobacterium violaceum ATCC 12472, Vibrio parahaemolyticus, Vibrio harveyi, Vibrio alginolyticus, Vibrio cholerae, and Proteus vulgaris. At biofilm inhibitory concentrations, biofilm formation was reduced by up to 70-90%. Furthermore, the potential quorum-sensing activity of the leaf extract was tested by agar well diffusion using Chromobacterium violaceum (ATCC 12472 & CV O26) reporter strains. The inhibition of violacein production may be due to direct or indirect interference on QS by active constituents or the interactive effect of different phytocompounds present in the extracts. This is the first report on antibiofilm and QS activity of D. falcata leaf extracts, signifying the scope for development of complementary medicine for biofilm-associated infections.
KW - bacterial pathogens
KW - biofilm
KW - confocal laser scanning microscopy
KW - Dendrophthoe falcata
KW - Loranthaceae
KW - quorum sensing
UR - http://www.scopus.com/inward/record.url?scp=84871610758&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84871610758&partnerID=8YFLogxK
U2 - 10.1055/s-0032-1327879
DO - 10.1055/s-0032-1327879
M3 - Article
VL - 78
SP - 1918
EP - 1926
JO - Planta Medica
JF - Planta Medica
SN - 0032-0943
IS - 18
ER -