Antibiofilm activity of dendrophthoe falcata against different bacterial pathogens

Alagarsamy Karthikeyan, Ramakrishnan Rameshkumar, Nallusamy Sivakumar, Issa S. Al Amri, Shunmugiah Karutha Pandian, Manikandan Ramesh

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Dendrophthoe falcata is a hemiparasitic plant commonly used for ailments such as ulcers, asthma, impotence, paralysis, skin diseases, menstrual troubles, pulmonary tuberculosis, and wounds. In this context, the validations of the traditional claim that the leaf extract of D. falcata possesses antibiofilm and anti-quorum sensing activity against different bacterial pathogens were assessed. The bacterial biofilms were quantified by crystal violet staining. Among the 17 bacterial pathogens screened, the methanolic fraction of the leaf extract clearly demonstrated antibiofilm activity for Proteus mirabilis, Vibrio vulnificus, Aeromonas hydrophila, Shigella sonnei, Chromobacterium violaceum ATCC 12472, Vibrio parahaemolyticus, Vibrio harveyi, Vibrio alginolyticus, Vibrio cholerae, and Proteus vulgaris. At biofilm inhibitory concentrations, biofilm formation was reduced by up to 70-90%. Furthermore, the potential quorum-sensing activity of the leaf extract was tested by agar well diffusion using Chromobacterium violaceum (ATCC 12472 & CV O26) reporter strains. The inhibition of violacein production may be due to direct or indirect interference on QS by active constituents or the interactive effect of different phytocompounds present in the extracts. This is the first report on antibiofilm and QS activity of D. falcata leaf extracts, signifying the scope for development of complementary medicine for biofilm-associated infections.

Original languageEnglish
Pages (from-to)1918-1926
Number of pages9
JournalPlanta Medica
Volume78
Issue number18
DOIs
Publication statusPublished - 2012

Fingerprint

Biofilms
Pathogens
Chromobacterium
Quorum Sensing
Vibrio alginolyticus
Vibrio vulnificus
Shigella sonnei
Proteus vulgaris
Vibrio parahaemolyticus
Gentian Violet
Aeromonas hydrophila
Proteus mirabilis
Vibrio cholerae
Vibrio
Erectile Dysfunction
Complementary Therapies
Pulmonary Tuberculosis
Skin Diseases
Paralysis
Ulcer

Keywords

  • bacterial pathogens
  • biofilm
  • confocal laser scanning microscopy
  • Dendrophthoe falcata
  • Loranthaceae
  • quorum sensing

ASJC Scopus subject areas

  • Complementary and alternative medicine
  • Molecular Medicine
  • Organic Chemistry
  • Analytical Chemistry
  • Pharmaceutical Science
  • Pharmacology
  • Drug Discovery

Cite this

Karthikeyan, A., Rameshkumar, R., Sivakumar, N., Al Amri, I. S., Karutha Pandian, S., & Ramesh, M. (2012). Antibiofilm activity of dendrophthoe falcata against different bacterial pathogens. Planta Medica, 78(18), 1918-1926. https://doi.org/10.1055/s-0032-1327879

Antibiofilm activity of dendrophthoe falcata against different bacterial pathogens. / Karthikeyan, Alagarsamy; Rameshkumar, Ramakrishnan; Sivakumar, Nallusamy; Al Amri, Issa S.; Karutha Pandian, Shunmugiah; Ramesh, Manikandan.

In: Planta Medica, Vol. 78, No. 18, 2012, p. 1918-1926.

Research output: Contribution to journalArticle

Karthikeyan, A, Rameshkumar, R, Sivakumar, N, Al Amri, IS, Karutha Pandian, S & Ramesh, M 2012, 'Antibiofilm activity of dendrophthoe falcata against different bacterial pathogens', Planta Medica, vol. 78, no. 18, pp. 1918-1926. https://doi.org/10.1055/s-0032-1327879
Karthikeyan A, Rameshkumar R, Sivakumar N, Al Amri IS, Karutha Pandian S, Ramesh M. Antibiofilm activity of dendrophthoe falcata against different bacterial pathogens. Planta Medica. 2012;78(18):1918-1926. https://doi.org/10.1055/s-0032-1327879
Karthikeyan, Alagarsamy ; Rameshkumar, Ramakrishnan ; Sivakumar, Nallusamy ; Al Amri, Issa S. ; Karutha Pandian, Shunmugiah ; Ramesh, Manikandan. / Antibiofilm activity of dendrophthoe falcata against different bacterial pathogens. In: Planta Medica. 2012 ; Vol. 78, No. 18. pp. 1918-1926.
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