Analysis of variability among the isolates of Peronosclerospora sorghi from sorghum and cornbased on restriction fragment length polymorphismof ITS region of ribosomal DNA

S. Mathiyazhagan, M. Karthikeyan, R. Sandosskumar, R. Velazhahan

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1 Citation (Scopus)

Abstract

Downy mildew, caused by Peronosclerospora sorghi [Weston and Uppal (Shaw)], is one of the most serious diseases of sorghum and corn worldwide. Pathogenic variability among various isolates of P. sorghi has been reported. However, the molecular relationships between the isolates from sorghum and corn are unknown. In the present study DNA was extracted from P. sorghi isolates from sorghum and corn collected from different locations of Tamil Nadu, India, and their genetic variability was investigated using restriction fragment length polymorphism (RFLP) analysis of the PCR-amplified internal transcribed spacer (ITS) region of ribosomal DNA. PCR amplification of the ITS regions of the P. sorghi isolates from sorghum and corn gave products of approximately 550 base pairs in length with slight variations in a few isolates. Restriction fragment length polymorphism analysis of the ITS regions of nuclear rDNA of P. sorghi with HhaI, EcoRI and MspI confirmed the heterogeneity among the P. sorghi isolates from sorghum and corn.

Original languageEnglish
Pages (from-to)31-37
Number of pages7
JournalArchives of Phytopathology and Plant Protection
Volume41
Issue number1
DOIs
Publication statusPublished - Feb 2008

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Peronosclerospora sorghi
ribosomal DNA
internal transcribed spacers
corn
restriction fragment length polymorphism
India
downy mildew
genetic variation
DNA

Keywords

  • Peronosclerospora sorghi
  • RFLPs
  • Ribosomal DNA internal transcribed spacer (ITS) region

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

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title = "Analysis of variability among the isolates of Peronosclerospora sorghi from sorghum and cornbased on restriction fragment length polymorphismof ITS region of ribosomal DNA",
abstract = "Downy mildew, caused by Peronosclerospora sorghi [Weston and Uppal (Shaw)], is one of the most serious diseases of sorghum and corn worldwide. Pathogenic variability among various isolates of P. sorghi has been reported. However, the molecular relationships between the isolates from sorghum and corn are unknown. In the present study DNA was extracted from P. sorghi isolates from sorghum and corn collected from different locations of Tamil Nadu, India, and their genetic variability was investigated using restriction fragment length polymorphism (RFLP) analysis of the PCR-amplified internal transcribed spacer (ITS) region of ribosomal DNA. PCR amplification of the ITS regions of the P. sorghi isolates from sorghum and corn gave products of approximately 550 base pairs in length with slight variations in a few isolates. Restriction fragment length polymorphism analysis of the ITS regions of nuclear rDNA of P. sorghi with HhaI, EcoRI and MspI confirmed the heterogeneity among the P. sorghi isolates from sorghum and corn.",
keywords = "Peronosclerospora sorghi, RFLPs, Ribosomal DNA internal transcribed spacer (ITS) region",
author = "S. Mathiyazhagan and M. Karthikeyan and R. Sandosskumar and R. Velazhahan",
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AU - Mathiyazhagan, S.

AU - Karthikeyan, M.

AU - Sandosskumar, R.

AU - Velazhahan, R.

PY - 2008/2

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N2 - Downy mildew, caused by Peronosclerospora sorghi [Weston and Uppal (Shaw)], is one of the most serious diseases of sorghum and corn worldwide. Pathogenic variability among various isolates of P. sorghi has been reported. However, the molecular relationships between the isolates from sorghum and corn are unknown. In the present study DNA was extracted from P. sorghi isolates from sorghum and corn collected from different locations of Tamil Nadu, India, and their genetic variability was investigated using restriction fragment length polymorphism (RFLP) analysis of the PCR-amplified internal transcribed spacer (ITS) region of ribosomal DNA. PCR amplification of the ITS regions of the P. sorghi isolates from sorghum and corn gave products of approximately 550 base pairs in length with slight variations in a few isolates. Restriction fragment length polymorphism analysis of the ITS regions of nuclear rDNA of P. sorghi with HhaI, EcoRI and MspI confirmed the heterogeneity among the P. sorghi isolates from sorghum and corn.

AB - Downy mildew, caused by Peronosclerospora sorghi [Weston and Uppal (Shaw)], is one of the most serious diseases of sorghum and corn worldwide. Pathogenic variability among various isolates of P. sorghi has been reported. However, the molecular relationships between the isolates from sorghum and corn are unknown. In the present study DNA was extracted from P. sorghi isolates from sorghum and corn collected from different locations of Tamil Nadu, India, and their genetic variability was investigated using restriction fragment length polymorphism (RFLP) analysis of the PCR-amplified internal transcribed spacer (ITS) region of ribosomal DNA. PCR amplification of the ITS regions of the P. sorghi isolates from sorghum and corn gave products of approximately 550 base pairs in length with slight variations in a few isolates. Restriction fragment length polymorphism analysis of the ITS regions of nuclear rDNA of P. sorghi with HhaI, EcoRI and MspI confirmed the heterogeneity among the P. sorghi isolates from sorghum and corn.

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