Analysis of phenylephrine hydrochloride in pharmaceutical formulations and biological fluids using (2,2′-bipyridyl)ruthenium(ii)-peroxydisulphate chemiluminescence system in a two-chip microdevice

Haider A J Al Lawati, Mahmood Al-Azwani, Gouri B. Varma, Fakhr Eldin O Suliman, Khaled A. Shalabi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A two-chip microdevice has been developed for the analysis of phenylephrine hydrochloride (PEH) in pharmaceutical formulations and biological fluids using (2,2′-bipyridyl)ruthenium(ii) (Ru(bipy) 3 2+)- peroxydisulphate chemiluminescence (CL) system. It was observed that a pre-derivatization step is required to obtain an analytically useful CL signal. Using 0.5 mol L -1 formaldehyde solution as derivatization reagent, eighteen-time enhancement in the CL signal was observed. The derivatization reaction, photoreaction of (Ru(bipy) 3 2+) with peroxydisulphate to produce (Ru(bipy) 3 3+), reaction of (Ru(bipy) 3 3+) with the derivatization product and detection of the produced CL signal were all carried out in this two-chip microdevice using minute quantity of reagents (27 μL per run). Various parameters that influence the derivatization reaction and the CL signal intensity were optimized. These include pH, flow rates and concentration of reagents used. A linear response was observed over the range 0.25 μg mL -1 to 15.0 μg mL -1 (R 2 = 0.9999) with RSD values of 0.03% (n = 15) for 1 μg mL -1. The limit of detection (LOD) was found to be 0.027 μg mL -1 (S/N = 3). The developed method was successfully applied for the analysis of PEH in biological fluids and pharmaceutical formulations.

Original languageEnglish
Pages (from-to)2585-2592
Number of pages8
JournalAnalytical Methods
Volume3
Issue number11
DOIs
Publication statusPublished - Nov 2011

Fingerprint

2,2'-Dipyridyl
Chemiluminescence
Ruthenium
Phenylephrine
Drug products
Fluids
Pharmaceutical Preparations
Formaldehyde
Flow rate

ASJC Scopus subject areas

  • Analytical Chemistry
  • Engineering(all)
  • Chemical Engineering(all)

Cite this

@article{e6365e9130bf4f5696756d815f3912a6,
title = "Analysis of phenylephrine hydrochloride in pharmaceutical formulations and biological fluids using (2,2′-bipyridyl)ruthenium(ii)-peroxydisulphate chemiluminescence system in a two-chip microdevice",
abstract = "A two-chip microdevice has been developed for the analysis of phenylephrine hydrochloride (PEH) in pharmaceutical formulations and biological fluids using (2,2′-bipyridyl)ruthenium(ii) (Ru(bipy) 3 2+)- peroxydisulphate chemiluminescence (CL) system. It was observed that a pre-derivatization step is required to obtain an analytically useful CL signal. Using 0.5 mol L -1 formaldehyde solution as derivatization reagent, eighteen-time enhancement in the CL signal was observed. The derivatization reaction, photoreaction of (Ru(bipy) 3 2+) with peroxydisulphate to produce (Ru(bipy) 3 3+), reaction of (Ru(bipy) 3 3+) with the derivatization product and detection of the produced CL signal were all carried out in this two-chip microdevice using minute quantity of reagents (27 μL per run). Various parameters that influence the derivatization reaction and the CL signal intensity were optimized. These include pH, flow rates and concentration of reagents used. A linear response was observed over the range 0.25 μg mL -1 to 15.0 μg mL -1 (R 2 = 0.9999) with RSD values of 0.03{\%} (n = 15) for 1 μg mL -1. The limit of detection (LOD) was found to be 0.027 μg mL -1 (S/N = 3). The developed method was successfully applied for the analysis of PEH in biological fluids and pharmaceutical formulations.",
author = "{Al Lawati}, {Haider A J} and Mahmood Al-Azwani and Varma, {Gouri B.} and Suliman, {Fakhr Eldin O} and Shalabi, {Khaled A.}",
year = "2011",
month = "11",
doi = "10.1039/c1ay05346a",
language = "English",
volume = "3",
pages = "2585--2592",
journal = "Analytical Methods",
issn = "1759-9660",
publisher = "Royal Society of Chemistry",
number = "11",

}

TY - JOUR

T1 - Analysis of phenylephrine hydrochloride in pharmaceutical formulations and biological fluids using (2,2′-bipyridyl)ruthenium(ii)-peroxydisulphate chemiluminescence system in a two-chip microdevice

AU - Al Lawati, Haider A J

AU - Al-Azwani, Mahmood

AU - Varma, Gouri B.

AU - Suliman, Fakhr Eldin O

AU - Shalabi, Khaled A.

PY - 2011/11

Y1 - 2011/11

N2 - A two-chip microdevice has been developed for the analysis of phenylephrine hydrochloride (PEH) in pharmaceutical formulations and biological fluids using (2,2′-bipyridyl)ruthenium(ii) (Ru(bipy) 3 2+)- peroxydisulphate chemiluminescence (CL) system. It was observed that a pre-derivatization step is required to obtain an analytically useful CL signal. Using 0.5 mol L -1 formaldehyde solution as derivatization reagent, eighteen-time enhancement in the CL signal was observed. The derivatization reaction, photoreaction of (Ru(bipy) 3 2+) with peroxydisulphate to produce (Ru(bipy) 3 3+), reaction of (Ru(bipy) 3 3+) with the derivatization product and detection of the produced CL signal were all carried out in this two-chip microdevice using minute quantity of reagents (27 μL per run). Various parameters that influence the derivatization reaction and the CL signal intensity were optimized. These include pH, flow rates and concentration of reagents used. A linear response was observed over the range 0.25 μg mL -1 to 15.0 μg mL -1 (R 2 = 0.9999) with RSD values of 0.03% (n = 15) for 1 μg mL -1. The limit of detection (LOD) was found to be 0.027 μg mL -1 (S/N = 3). The developed method was successfully applied for the analysis of PEH in biological fluids and pharmaceutical formulations.

AB - A two-chip microdevice has been developed for the analysis of phenylephrine hydrochloride (PEH) in pharmaceutical formulations and biological fluids using (2,2′-bipyridyl)ruthenium(ii) (Ru(bipy) 3 2+)- peroxydisulphate chemiluminescence (CL) system. It was observed that a pre-derivatization step is required to obtain an analytically useful CL signal. Using 0.5 mol L -1 formaldehyde solution as derivatization reagent, eighteen-time enhancement in the CL signal was observed. The derivatization reaction, photoreaction of (Ru(bipy) 3 2+) with peroxydisulphate to produce (Ru(bipy) 3 3+), reaction of (Ru(bipy) 3 3+) with the derivatization product and detection of the produced CL signal were all carried out in this two-chip microdevice using minute quantity of reagents (27 μL per run). Various parameters that influence the derivatization reaction and the CL signal intensity were optimized. These include pH, flow rates and concentration of reagents used. A linear response was observed over the range 0.25 μg mL -1 to 15.0 μg mL -1 (R 2 = 0.9999) with RSD values of 0.03% (n = 15) for 1 μg mL -1. The limit of detection (LOD) was found to be 0.027 μg mL -1 (S/N = 3). The developed method was successfully applied for the analysis of PEH in biological fluids and pharmaceutical formulations.

UR - http://www.scopus.com/inward/record.url?scp=84255167888&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84255167888&partnerID=8YFLogxK

U2 - 10.1039/c1ay05346a

DO - 10.1039/c1ay05346a

M3 - Article

VL - 3

SP - 2585

EP - 2592

JO - Analytical Methods

JF - Analytical Methods

SN - 1759-9660

IS - 11

ER -