Amelioration of azoxymethane induced-carcinogenesis by reducing oxidative stress in rat colon by natural extracts

Mostafa I. Waly, Amani S. Al-Rawahi, Marwa Al Riyami, Mohamed A. Al-Kindi, Halima K. Al-Issaei, Sardar A. Farooq, Ahmed Al-Alawi, Mohammad S. Rahman

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Abstract

Background: Azoxymethane (AOM) is a potent carcinogenic agent commonly used to induce colon cancer in rats; the cytotoxicity of AOM is considered to mediate oxidative stress. This study investigated the chemopreventive effect of three natural extracts [pomegranate peel extract (PomPE), papaya peel extract (PapPE) and seaweed extract (SE)] against AOM-induced oxidative stress and carcinogenesis in rat colon.Methods: Eighty Sprague-Dawley rats (aged 4 weeks) were randomly divided into 8 groups (10 rats/group). Control group was fed a basal diet; AOM-treated group was fed a basal diet and received AOM intraperitonial injections for two weeks at a dose of 15 mg/kg bodyweight, whereas the other six groups were received oral supplementation of PomPE, PapPE or SE, in the presence or absence of AOM injection. All animals were continuously fed ad-libitum until aged 16 weeks, then all rats were sacrificed and the colon tissues were examined microscopically for pathological changes and aberrant crypt foci (ACF) development, genotoxicity (induced micronuclei (MN) cells enumeration), and glutathione and lipid peroxidation.Results: Our results showed that AOM-induced ACF development and pathological changes in the colonic mucosal tissues, increased bone marrow MN cells and oxidative stress (glutathione depletion, lipid peroxidation) in rat colonic cells. The concomitant treatment of AOM with PomPE, PapPE or SE significantly ameliorated the cytotoxic effects of AOM.Conclusions: The results of this study provide in-vivo evidence that PomPE, PapPE and SE reduced the AOM-induced colon cancer in rats, through their potent anti-oxidant activities.

Original languageEnglish
Article number60
JournalBMC Complementary and Alternative Medicine
Volume14
DOIs
Publication statusPublished - Feb 18 2014

Fingerprint

Azoxymethane
Colon
Carcinogenesis
Oxidative Stress
Punicaceae
Carica
Seaweed
Aberrant Crypt Foci
Colonic Neoplasms
Lipid Peroxidation
Glutathione
Diet
Injections
Oxidants
Bone Marrow Cells
Sprague Dawley Rats
Mucous Membrane

Keywords

  • Azoxymethane
  • Colon cancer
  • Oxidative stress

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

@article{9f0791f7a33d451292e73c8cc6f6ac7e,
title = "Amelioration of azoxymethane induced-carcinogenesis by reducing oxidative stress in rat colon by natural extracts",
abstract = "Background: Azoxymethane (AOM) is a potent carcinogenic agent commonly used to induce colon cancer in rats; the cytotoxicity of AOM is considered to mediate oxidative stress. This study investigated the chemopreventive effect of three natural extracts [pomegranate peel extract (PomPE), papaya peel extract (PapPE) and seaweed extract (SE)] against AOM-induced oxidative stress and carcinogenesis in rat colon.Methods: Eighty Sprague-Dawley rats (aged 4 weeks) were randomly divided into 8 groups (10 rats/group). Control group was fed a basal diet; AOM-treated group was fed a basal diet and received AOM intraperitonial injections for two weeks at a dose of 15 mg/kg bodyweight, whereas the other six groups were received oral supplementation of PomPE, PapPE or SE, in the presence or absence of AOM injection. All animals were continuously fed ad-libitum until aged 16 weeks, then all rats were sacrificed and the colon tissues were examined microscopically for pathological changes and aberrant crypt foci (ACF) development, genotoxicity (induced micronuclei (MN) cells enumeration), and glutathione and lipid peroxidation.Results: Our results showed that AOM-induced ACF development and pathological changes in the colonic mucosal tissues, increased bone marrow MN cells and oxidative stress (glutathione depletion, lipid peroxidation) in rat colonic cells. The concomitant treatment of AOM with PomPE, PapPE or SE significantly ameliorated the cytotoxic effects of AOM.Conclusions: The results of this study provide in-vivo evidence that PomPE, PapPE and SE reduced the AOM-induced colon cancer in rats, through their potent anti-oxidant activities.",
keywords = "Azoxymethane, Colon cancer, Oxidative stress",
author = "Waly, {Mostafa I.} and Al-Rawahi, {Amani S.} and {Al Riyami}, Marwa and Al-Kindi, {Mohamed A.} and Al-Issaei, {Halima K.} and Farooq, {Sardar A.} and Ahmed Al-Alawi and Rahman, {Mohammad S.}",
year = "2014",
month = "2",
day = "18",
doi = "10.1186/1472-6882-14-60",
language = "English",
volume = "14",
journal = "BMC Complementary and Alternative Medicine",
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TY - JOUR

T1 - Amelioration of azoxymethane induced-carcinogenesis by reducing oxidative stress in rat colon by natural extracts

AU - Waly, Mostafa I.

AU - Al-Rawahi, Amani S.

AU - Al Riyami, Marwa

AU - Al-Kindi, Mohamed A.

AU - Al-Issaei, Halima K.

AU - Farooq, Sardar A.

AU - Al-Alawi, Ahmed

AU - Rahman, Mohammad S.

PY - 2014/2/18

Y1 - 2014/2/18

N2 - Background: Azoxymethane (AOM) is a potent carcinogenic agent commonly used to induce colon cancer in rats; the cytotoxicity of AOM is considered to mediate oxidative stress. This study investigated the chemopreventive effect of three natural extracts [pomegranate peel extract (PomPE), papaya peel extract (PapPE) and seaweed extract (SE)] against AOM-induced oxidative stress and carcinogenesis in rat colon.Methods: Eighty Sprague-Dawley rats (aged 4 weeks) were randomly divided into 8 groups (10 rats/group). Control group was fed a basal diet; AOM-treated group was fed a basal diet and received AOM intraperitonial injections for two weeks at a dose of 15 mg/kg bodyweight, whereas the other six groups were received oral supplementation of PomPE, PapPE or SE, in the presence or absence of AOM injection. All animals were continuously fed ad-libitum until aged 16 weeks, then all rats were sacrificed and the colon tissues were examined microscopically for pathological changes and aberrant crypt foci (ACF) development, genotoxicity (induced micronuclei (MN) cells enumeration), and glutathione and lipid peroxidation.Results: Our results showed that AOM-induced ACF development and pathological changes in the colonic mucosal tissues, increased bone marrow MN cells and oxidative stress (glutathione depletion, lipid peroxidation) in rat colonic cells. The concomitant treatment of AOM with PomPE, PapPE or SE significantly ameliorated the cytotoxic effects of AOM.Conclusions: The results of this study provide in-vivo evidence that PomPE, PapPE and SE reduced the AOM-induced colon cancer in rats, through their potent anti-oxidant activities.

AB - Background: Azoxymethane (AOM) is a potent carcinogenic agent commonly used to induce colon cancer in rats; the cytotoxicity of AOM is considered to mediate oxidative stress. This study investigated the chemopreventive effect of three natural extracts [pomegranate peel extract (PomPE), papaya peel extract (PapPE) and seaweed extract (SE)] against AOM-induced oxidative stress and carcinogenesis in rat colon.Methods: Eighty Sprague-Dawley rats (aged 4 weeks) were randomly divided into 8 groups (10 rats/group). Control group was fed a basal diet; AOM-treated group was fed a basal diet and received AOM intraperitonial injections for two weeks at a dose of 15 mg/kg bodyweight, whereas the other six groups were received oral supplementation of PomPE, PapPE or SE, in the presence or absence of AOM injection. All animals were continuously fed ad-libitum until aged 16 weeks, then all rats were sacrificed and the colon tissues were examined microscopically for pathological changes and aberrant crypt foci (ACF) development, genotoxicity (induced micronuclei (MN) cells enumeration), and glutathione and lipid peroxidation.Results: Our results showed that AOM-induced ACF development and pathological changes in the colonic mucosal tissues, increased bone marrow MN cells and oxidative stress (glutathione depletion, lipid peroxidation) in rat colonic cells. The concomitant treatment of AOM with PomPE, PapPE or SE significantly ameliorated the cytotoxic effects of AOM.Conclusions: The results of this study provide in-vivo evidence that PomPE, PapPE and SE reduced the AOM-induced colon cancer in rats, through their potent anti-oxidant activities.

KW - Azoxymethane

KW - Colon cancer

KW - Oxidative stress

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U2 - 10.1186/1472-6882-14-60

DO - 10.1186/1472-6882-14-60

M3 - Article

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JO - BMC Complementary and Alternative Medicine

JF - BMC Complementary and Alternative Medicine

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