Amelioration of Aluminum Maltolate-Induced Inflammation and Endoplasmic Reticulum Stress-Mediated Apoptosis by Tannoid Principles of Emblica officinalis in Neuronal Cellular Model

Mathiyazahan Dhivya Bharathi, Arokiasamy Justin-Thenmozhi, Thamilarasan Manivasagam, Mashoque Ahmad Rather, Chidambaram Saravana Babu, Mohamed Musthafa Essa, Gilles J. Guillemin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The neuroprotective role of tannoid principles of Emblica officinalis (EoT), an Indian and Chinese traditional medicinal plant against memory loss in aluminum chloride-induced in vivo model of Alzheimer’s disease through attenuating AChE activity, oxidative stress, amyloid and tau toxicity, and apoptosis, was recently reported in our lab. However, to further elucidate the mechanism of neuroprotective effect of EoT, the current study was designed to evaluate endoplasmic reticulum stress-suppressing and anti-inflammatory role of EoT in PC 12 and SH-SY 5Y cells. These cells were divided into four groups: control (aluminum maltolate (Al(mal)3), EoT + Al(mal)3, and EoT alone based on 3-(4, 5-dimethyl 2-yl)-2, and 5-diphenyltetrazolium bromide (MTT) assay. EoT significantly reduced Al(mal)3-induced cell death and attenuated ROS, mitochondrial membrane dysfunction, and apoptosis (protein expressions of Bax; Bcl-2; cleaved caspases 3, 6, 9, 12; and cytochrome c) by regulating endoplasmic reticulum stress (PKR-like ER kinase (PERK), α subunit of eukaryotic initiation factor 2 (EIF2-α), C/EBP-homologous protein (CHOP), and high-mobility group box 1 protein (HMGB1)). Moreover, inflammatory response (NF-κB, IL-1β, IL-6, and TNF-α) and Aβ toxicity (Aβ1–42) triggered by Al(mal)3 was significantly normalized by EoT. Our results suggested that EoT could be a possible/promising and novel therapeutic lead against Al-induced neurotoxicity. However, further extensive research is needed to prove its efficacy in clinical studies.

Original languageEnglish
JournalNeurotoxicity Research
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

Phyllanthus emblica
Endoplasmic Reticulum Stress
Apoptosis
Inflammation
Toxicity
Transcription Factor CHOP
Eukaryotic Initiation Factor-2
Caspase 6
HMGB1 Protein
bcl-2-Associated X Protein
Oxidative stress
Memory Disorders
Mitochondrial Membranes
Neuroprotective Agents
Cell death
Medicinal Plants
Cytochromes c
Bromides
Interleukin-1
Amyloid

Keywords

  • Aluminum maltolate
  • Endoplasmic reticulum stress
  • Inflammation
  • Neurotoxicity
  • Tannoids principles of Emblica officinalis

ASJC Scopus subject areas

  • Neuroscience(all)
  • Toxicology

Cite this

Amelioration of Aluminum Maltolate-Induced Inflammation and Endoplasmic Reticulum Stress-Mediated Apoptosis by Tannoid Principles of Emblica officinalis in Neuronal Cellular Model. / Dhivya Bharathi, Mathiyazahan; Justin-Thenmozhi, Arokiasamy; Manivasagam, Thamilarasan; Ahmad Rather, Mashoque; Saravana Babu, Chidambaram; Essa, Mohamed Musthafa; Guillemin, Gilles J.

In: Neurotoxicity Research, 01.01.2018.

Research output: Contribution to journalArticle

Dhivya Bharathi, Mathiyazahan ; Justin-Thenmozhi, Arokiasamy ; Manivasagam, Thamilarasan ; Ahmad Rather, Mashoque ; Saravana Babu, Chidambaram ; Essa, Mohamed Musthafa ; Guillemin, Gilles J. / Amelioration of Aluminum Maltolate-Induced Inflammation and Endoplasmic Reticulum Stress-Mediated Apoptosis by Tannoid Principles of Emblica officinalis in Neuronal Cellular Model. In: Neurotoxicity Research. 2018.
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AU - Manivasagam, Thamilarasan

AU - Ahmad Rather, Mashoque

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