TY - JOUR
T1 - Alternaria alternata and Neocosmospora sp. from the medicinal plant Euphorbia larica exhibit antagonistic activity against Fusarium sp., a plant pathogenic fungus
AU - Khuseib Hamed Al-Rashdi, Fatma
AU - Al-Sadi, Abdullah Mohammed
AU - Al-Riyamy, Bahja Z.
AU - S. N. Maharachchikumbura, Sajeewa
AU - Khalfan Al-Ruqaishi, Huda
AU - Velazhahan, Rethinasamy
N1 - Funding Information:
This study was supported by Sultan Qaboos University through the projects EG/AGR/CROP/16/01, CR/AGR/CROP/19/01 and IG/AGR/CROP/18/01. We thank Dr. Khalid Al-Farsi and Dr. Annette Patzelt, Oman Botanic Garden for providing Euphorbia larica and Dr. Jamal Nasser Al-Sabahi, CAMS, SQU for helping in GC-MS analysis.
Publisher Copyright:
© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - In this study, endophytic fungi were isolated from Euphorbia larica Boiss. and their antagonistic potential against phytopathogenic fungi viz. Fusarium sp., Rosellinia sanctae-cruciana and Cladosporium sp. was evaluated using an in vitro confrontation assay. A total of four endophytic fungi were isolated from the roots of E. larica. Among them, two fungal isolates (SQUCC-F1-1 and SQUCC-F6-2) showed inhibitory activity against Fusarium sp. None of the fungal endophytes restricted the growth of Rosellinia sanctae-cruciana and Cladosporium sp. On the basis of multi-gene phylogenetic analysis, these two endophytic fungi were identified as Neocosmospora sp. (SQUCC-F1-1) and Alternaria alternata (SQUCC-F6-2). Scanning electron microscopic observation of hyphae of the phytopathogenic fungi from the dual culture plate revealed morphological abnormalities such as loss of turgidity, shrinkage and disintegration of the hyphae. Analysis of the metabolites produced by these endophytic fungi by GC-MS revealed the existence of various fatty acids, fatty acid methyl esters, hydrocarbons and alkanes, which have been previously demonstrated to have antimicrobial activity. The antagonistic activities of fungal endophytes from E. larica are reported for the first time.
AB - In this study, endophytic fungi were isolated from Euphorbia larica Boiss. and their antagonistic potential against phytopathogenic fungi viz. Fusarium sp., Rosellinia sanctae-cruciana and Cladosporium sp. was evaluated using an in vitro confrontation assay. A total of four endophytic fungi were isolated from the roots of E. larica. Among them, two fungal isolates (SQUCC-F1-1 and SQUCC-F6-2) showed inhibitory activity against Fusarium sp. None of the fungal endophytes restricted the growth of Rosellinia sanctae-cruciana and Cladosporium sp. On the basis of multi-gene phylogenetic analysis, these two endophytic fungi were identified as Neocosmospora sp. (SQUCC-F1-1) and Alternaria alternata (SQUCC-F6-2). Scanning electron microscopic observation of hyphae of the phytopathogenic fungi from the dual culture plate revealed morphological abnormalities such as loss of turgidity, shrinkage and disintegration of the hyphae. Analysis of the metabolites produced by these endophytic fungi by GC-MS revealed the existence of various fatty acids, fatty acid methyl esters, hydrocarbons and alkanes, which have been previously demonstrated to have antimicrobial activity. The antagonistic activities of fungal endophytes from E. larica are reported for the first time.
KW - Alternaria alternata
KW - endophytes
KW - Euphorbiaceae
KW - Fusarium sp
KW - Neocosmospora sp
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U2 - 10.1080/26895293.2020.1759702
DO - 10.1080/26895293.2020.1759702
M3 - Article
AN - SCOPUS:85086446936
SN - 2689-5293
VL - 13
SP - 223
EP - 232
JO - All Life
JF - All Life
IS - 1
ER -