Altered gene expression may underlie prolonged duration of the QT interval and ventricular action potential in streptozotocin-induced diabetic rat heart

F. C. Howarth; M. Jacobson; M. A. Qureshi; M. Shafiullah; R. S. Hameed; E. Zilahi; A. Al Haj; N. Nowotny; E. Adeghate

doi:10.1007/s11010-009-0074-9

Altered gene expression may underlie prolonged duration of the QT interval and ventricular action potential in streptozotocin-induced diabetic rat heart

F. C. Howarth^*, M. Jacobson, M. A. Qureshi, M. Shafiullah, R. S. Hameed, E. Zilahi, A. Al Haj, N. Nowotny, E. Adeghate

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

Ventricular electrical conduction has been investigated in the streptozotocin (STZ)-induced diabetic rat. Diabetes was induced with a single injection of STZ (60 mg/kg bodyweight, ip). The ECG was measured continuously, in vivo, using a biotelemetry system. Left ventricular action potentials were recorded with an extracellular suction electrode. Expression of mRNA transcripts for selected ion transport proteins was measured in left ventricle with real-time RT-PCR. At 10 weeks after STZ treatment, in vivo heart rate (HR) was reduced (267 ± 3 vs. 329 ± 5 BPM), QRS complex duration and QT interval were prolonged in diabetic rats compared to controls. In vitro spontaneous HR was reduced and paced heart action potential repolarization was prolonged in diabetic rats compared to controls. The mRNA expression for Kcnd2 (I_to channel) and Kcne2 (I_kr channel) was significantly reduced in diabetic rats compared to controls. Altered gene expression and, in particular, genes that encode K⁺ channel proteins may underlie delayed propagation of electrical activity in the ventricular myocardium of STZ-induced diabetic rat.

Original language	English
Pages (from-to)	57-65
Number of pages	9
Journal	Molecular and Cellular Biochemistry
Volume	328
Issue number	1-2
DOIs	https://doi.org/10.1007/s11010-009-0074-9
Publication status	Published - 2009
Externally published	Yes

Keywords

Arrhythmias
Diabetes mellitus
Electrical activity
Heart

ASJC Scopus subject areas

Molecular Biology
Clinical Biochemistry
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s11010-009-0074-9

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Howarth, F. C., Jacobson, M., Qureshi, M. A., Shafiullah, M., Hameed, R. S., Zilahi, E., Al Haj, A., Nowotny, N., & Adeghate, E. (2009). Altered gene expression may underlie prolonged duration of the QT interval and ventricular action potential in streptozotocin-induced diabetic rat heart. Molecular and Cellular Biochemistry, 328(1-2), 57-65. https://doi.org/10.1007/s11010-009-0074-9

Howarth, FC, Jacobson, M, Qureshi, MA, Shafiullah, M, Hameed, RS, Zilahi, E, Al Haj, A, Nowotny, N & Adeghate, E 2009, 'Altered gene expression may underlie prolonged duration of the QT interval and ventricular action potential in streptozotocin-induced diabetic rat heart', Molecular and Cellular Biochemistry, vol. 328, no. 1-2, pp. 57-65. https://doi.org/10.1007/s11010-009-0074-9

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abstract = "Ventricular electrical conduction has been investigated in the streptozotocin (STZ)-induced diabetic rat. Diabetes was induced with a single injection of STZ (60 mg/kg bodyweight, ip). The ECG was measured continuously, in vivo, using a biotelemetry system. Left ventricular action potentials were recorded with an extracellular suction electrode. Expression of mRNA transcripts for selected ion transport proteins was measured in left ventricle with real-time RT-PCR. At 10 weeks after STZ treatment, in vivo heart rate (HR) was reduced (267 ± 3 vs. 329 ± 5 BPM), QRS complex duration and QT interval were prolonged in diabetic rats compared to controls. In vitro spontaneous HR was reduced and paced heart action potential repolarization was prolonged in diabetic rats compared to controls. The mRNA expression for Kcnd2 (Ito channel) and Kcne2 (Ikr channel) was significantly reduced in diabetic rats compared to controls. Altered gene expression and, in particular, genes that encode K+ channel proteins may underlie delayed propagation of electrical activity in the ventricular myocardium of STZ-induced diabetic rat.",

keywords = "Arrhythmias, Diabetes mellitus, Electrical activity, Heart",

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doi = "10.1007/s11010-009-0074-9",

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AU - Howarth, F. C.

AU - Jacobson, M.

AU - Qureshi, M. A.

AU - Shafiullah, M.

AU - Hameed, R. S.

AU - Zilahi, E.

AU - Al Haj, A.

AU - Nowotny, N.

AU - Adeghate, E.

N1 - Funding Information: Acknowledgements Project Grant from Faculty of Medicine & Health Sciences, UAE University

PY - 2009

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N2 - Ventricular electrical conduction has been investigated in the streptozotocin (STZ)-induced diabetic rat. Diabetes was induced with a single injection of STZ (60 mg/kg bodyweight, ip). The ECG was measured continuously, in vivo, using a biotelemetry system. Left ventricular action potentials were recorded with an extracellular suction electrode. Expression of mRNA transcripts for selected ion transport proteins was measured in left ventricle with real-time RT-PCR. At 10 weeks after STZ treatment, in vivo heart rate (HR) was reduced (267 ± 3 vs. 329 ± 5 BPM), QRS complex duration and QT interval were prolonged in diabetic rats compared to controls. In vitro spontaneous HR was reduced and paced heart action potential repolarization was prolonged in diabetic rats compared to controls. The mRNA expression for Kcnd2 (Ito channel) and Kcne2 (Ikr channel) was significantly reduced in diabetic rats compared to controls. Altered gene expression and, in particular, genes that encode K+ channel proteins may underlie delayed propagation of electrical activity in the ventricular myocardium of STZ-induced diabetic rat.

AB - Ventricular electrical conduction has been investigated in the streptozotocin (STZ)-induced diabetic rat. Diabetes was induced with a single injection of STZ (60 mg/kg bodyweight, ip). The ECG was measured continuously, in vivo, using a biotelemetry system. Left ventricular action potentials were recorded with an extracellular suction electrode. Expression of mRNA transcripts for selected ion transport proteins was measured in left ventricle with real-time RT-PCR. At 10 weeks after STZ treatment, in vivo heart rate (HR) was reduced (267 ± 3 vs. 329 ± 5 BPM), QRS complex duration and QT interval were prolonged in diabetic rats compared to controls. In vitro spontaneous HR was reduced and paced heart action potential repolarization was prolonged in diabetic rats compared to controls. The mRNA expression for Kcnd2 (Ito channel) and Kcne2 (Ikr channel) was significantly reduced in diabetic rats compared to controls. Altered gene expression and, in particular, genes that encode K+ channel proteins may underlie delayed propagation of electrical activity in the ventricular myocardium of STZ-induced diabetic rat.

KW - Arrhythmias

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KW - Electrical activity

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Altered gene expression may underlie prolonged duration of the QT interval and ventricular action potential in streptozotocin-induced diabetic rat heart

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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