Altered gene expression may underlie prolonged duration of the QT interval and ventricular action potential in streptozotocin-induced diabetic rat heart

F. C. Howarth, M. Jacobson, M. A. Qureshi, M. Shafiullah, R. S. Hameed, E. Zilahi, A. Al Haj, N. Nowotny, E. Adeghate

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Ventricular electrical conduction has been investigated in the streptozotocin (STZ)-induced diabetic rat. Diabetes was induced with a single injection of STZ (60 mg/kg bodyweight, ip). The ECG was measured continuously, in vivo, using a biotelemetry system. Left ventricular action potentials were recorded with an extracellular suction electrode. Expression of mRNA transcripts for selected ion transport proteins was measured in left ventricle with real-time RT-PCR. At 10 weeks after STZ treatment, in vivo heart rate (HR) was reduced (267 ± 3 vs. 329 ± 5 BPM), QRS complex duration and QT interval were prolonged in diabetic rats compared to controls. In vitro spontaneous HR was reduced and paced heart action potential repolarization was prolonged in diabetic rats compared to controls. The mRNA expression for Kcnd2 (Ito channel) and Kcne2 (Ikr channel) was significantly reduced in diabetic rats compared to controls. Altered gene expression and, in particular, genes that encode K+ channel proteins may underlie delayed propagation of electrical activity in the ventricular myocardium of STZ-induced diabetic rat.

Original languageEnglish
Pages (from-to)57-65
Number of pages9
JournalMolecular and Cellular Biochemistry
Volume328
Issue number1-2
DOIs
Publication statusPublished - 2009

Fingerprint

Streptozocin
Gene expression
Action Potentials
Rats
Gene Expression
Biotelemetry
Heart Rate
Messenger RNA
Ion Transport
Suction
Medical problems
Electrocardiography
Heart Ventricles
Real-Time Polymerase Chain Reaction
Myocardium
Carrier Proteins
Electrodes
Genes
Ions
Injections

Keywords

  • Arrhythmias
  • Diabetes mellitus
  • Electrical activity
  • Heart

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Altered gene expression may underlie prolonged duration of the QT interval and ventricular action potential in streptozotocin-induced diabetic rat heart. / Howarth, F. C.; Jacobson, M.; Qureshi, M. A.; Shafiullah, M.; Hameed, R. S.; Zilahi, E.; Al Haj, A.; Nowotny, N.; Adeghate, E.

In: Molecular and Cellular Biochemistry, Vol. 328, No. 1-2, 2009, p. 57-65.

Research output: Contribution to journalArticle

Howarth, F. C. ; Jacobson, M. ; Qureshi, M. A. ; Shafiullah, M. ; Hameed, R. S. ; Zilahi, E. ; Al Haj, A. ; Nowotny, N. ; Adeghate, E. / Altered gene expression may underlie prolonged duration of the QT interval and ventricular action potential in streptozotocin-induced diabetic rat heart. In: Molecular and Cellular Biochemistry. 2009 ; Vol. 328, No. 1-2. pp. 57-65.
@article{dba222429758457f98043b943af83816,
title = "Altered gene expression may underlie prolonged duration of the QT interval and ventricular action potential in streptozotocin-induced diabetic rat heart",
abstract = "Ventricular electrical conduction has been investigated in the streptozotocin (STZ)-induced diabetic rat. Diabetes was induced with a single injection of STZ (60 mg/kg bodyweight, ip). The ECG was measured continuously, in vivo, using a biotelemetry system. Left ventricular action potentials were recorded with an extracellular suction electrode. Expression of mRNA transcripts for selected ion transport proteins was measured in left ventricle with real-time RT-PCR. At 10 weeks after STZ treatment, in vivo heart rate (HR) was reduced (267 ± 3 vs. 329 ± 5 BPM), QRS complex duration and QT interval were prolonged in diabetic rats compared to controls. In vitro spontaneous HR was reduced and paced heart action potential repolarization was prolonged in diabetic rats compared to controls. The mRNA expression for Kcnd2 (Ito channel) and Kcne2 (Ikr channel) was significantly reduced in diabetic rats compared to controls. Altered gene expression and, in particular, genes that encode K+ channel proteins may underlie delayed propagation of electrical activity in the ventricular myocardium of STZ-induced diabetic rat.",
keywords = "Arrhythmias, Diabetes mellitus, Electrical activity, Heart",
author = "Howarth, {F. C.} and M. Jacobson and Qureshi, {M. A.} and M. Shafiullah and Hameed, {R. S.} and E. Zilahi and {Al Haj}, A. and N. Nowotny and E. Adeghate",
year = "2009",
doi = "10.1007/s11010-009-0074-9",
language = "English",
volume = "328",
pages = "57--65",
journal = "Molecular and Cellular Biochemistry",
issn = "0300-8177",
publisher = "Springer Netherlands",
number = "1-2",

}

TY - JOUR

T1 - Altered gene expression may underlie prolonged duration of the QT interval and ventricular action potential in streptozotocin-induced diabetic rat heart

AU - Howarth, F. C.

AU - Jacobson, M.

AU - Qureshi, M. A.

AU - Shafiullah, M.

AU - Hameed, R. S.

AU - Zilahi, E.

AU - Al Haj, A.

AU - Nowotny, N.

AU - Adeghate, E.

PY - 2009

Y1 - 2009

N2 - Ventricular electrical conduction has been investigated in the streptozotocin (STZ)-induced diabetic rat. Diabetes was induced with a single injection of STZ (60 mg/kg bodyweight, ip). The ECG was measured continuously, in vivo, using a biotelemetry system. Left ventricular action potentials were recorded with an extracellular suction electrode. Expression of mRNA transcripts for selected ion transport proteins was measured in left ventricle with real-time RT-PCR. At 10 weeks after STZ treatment, in vivo heart rate (HR) was reduced (267 ± 3 vs. 329 ± 5 BPM), QRS complex duration and QT interval were prolonged in diabetic rats compared to controls. In vitro spontaneous HR was reduced and paced heart action potential repolarization was prolonged in diabetic rats compared to controls. The mRNA expression for Kcnd2 (Ito channel) and Kcne2 (Ikr channel) was significantly reduced in diabetic rats compared to controls. Altered gene expression and, in particular, genes that encode K+ channel proteins may underlie delayed propagation of electrical activity in the ventricular myocardium of STZ-induced diabetic rat.

AB - Ventricular electrical conduction has been investigated in the streptozotocin (STZ)-induced diabetic rat. Diabetes was induced with a single injection of STZ (60 mg/kg bodyweight, ip). The ECG was measured continuously, in vivo, using a biotelemetry system. Left ventricular action potentials were recorded with an extracellular suction electrode. Expression of mRNA transcripts for selected ion transport proteins was measured in left ventricle with real-time RT-PCR. At 10 weeks after STZ treatment, in vivo heart rate (HR) was reduced (267 ± 3 vs. 329 ± 5 BPM), QRS complex duration and QT interval were prolonged in diabetic rats compared to controls. In vitro spontaneous HR was reduced and paced heart action potential repolarization was prolonged in diabetic rats compared to controls. The mRNA expression for Kcnd2 (Ito channel) and Kcne2 (Ikr channel) was significantly reduced in diabetic rats compared to controls. Altered gene expression and, in particular, genes that encode K+ channel proteins may underlie delayed propagation of electrical activity in the ventricular myocardium of STZ-induced diabetic rat.

KW - Arrhythmias

KW - Diabetes mellitus

KW - Electrical activity

KW - Heart

UR - http://www.scopus.com/inward/record.url?scp=67650255092&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67650255092&partnerID=8YFLogxK

U2 - 10.1007/s11010-009-0074-9

DO - 10.1007/s11010-009-0074-9

M3 - Article

VL - 328

SP - 57

EP - 65

JO - Molecular and Cellular Biochemistry

JF - Molecular and Cellular Biochemistry

SN - 0300-8177

IS - 1-2

ER -