Agaricus blazei extract attenuates rotenone-induced apoptosis through its mitochondrial protective and antioxidant properties in SH-SY5Y neuroblastoma cells

Veerappan Venkatesh Gobi, Srinivasagam Rajasankar*, Muthu Ramkumar, Chinnasamy Dhanalakshmi, Thamilarasan Manivasagam, Arokiasamy Justin Thenmozhi, Musthafa Mohamed Essa, Ranganathan Chidambaram

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

The present study was aimed to find out the effect of Agaricus blazei mushroom extract against rotenone-induced cellular model. SH-SY5Y neuroblastoma cells are divided into four experimental groups (control, rotenone (100 nM), A. blazei (5 μg/ml) + rotenone (100 nM), and A. blazei alone treated) based on MTT assay, cells were allowed to measure the ROS, TBARS levels, and antioxidants activities. Finally, mitochondrial transmembrane potential (MMP) and expressions of apoptotic proteins were also analyzed. Pre-treatment with A. blazei significantly enhanced cell viability, attenuated rotenone-induced ROS, MMP, and apoptosis. Our results indicated that anti-apoptotic properties of this natural compound due to its antioxidant and mitochondrial protective function protect rotenone-induced cytotoxicity. Therefore, it may be concluded that A. blazei can be further developed as a promising drug for the treatment of Parkinson’s disease (PD).

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalNutritional Neuroscience
DOIs
Publication statusAccepted/In press - Sep 20 2016

Keywords

  • A. blazei
  • Apoptosis
  • Mitochondria membrane potential
  • Oxidative stress
  • Rotenone

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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