Agaricus blazei extract attenuates rotenone-induced apoptosis through its mitochondrial protective and antioxidant properties in SH-SY5Y neuroblastoma cells

Veerappan Venkatesh Gobi, Srinivasagam Rajasankar, Muthu Ramkumar, Chinnasamy Dhanalakshmi, Thamilarasan Manivasagam, Arokiasamy Justin Thenmozhi, Musthafa Mohamed Essa, Ranganathan Chidambaram

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The present study was aimed to find out the effect of Agaricus blazei mushroom extract against rotenone-induced cellular model. SH-SY5Y neuroblastoma cells are divided into four experimental groups (control, rotenone (100 nM), A. blazei (5 μg/ml) + rotenone (100 nM), and A. blazei alone treated) based on MTT assay, cells were allowed to measure the ROS, TBARS levels, and antioxidants activities. Finally, mitochondrial transmembrane potential (MMP) and expressions of apoptotic proteins were also analyzed. Pre-treatment with A. blazei significantly enhanced cell viability, attenuated rotenone-induced ROS, MMP, and apoptosis. Our results indicated that anti-apoptotic properties of this natural compound due to its antioxidant and mitochondrial protective function protect rotenone-induced cytotoxicity. Therefore, it may be concluded that A. blazei can be further developed as a promising drug for the treatment of Parkinson’s disease (PD).

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalNutritional Neuroscience
DOIs
Publication statusAccepted/In press - Sep 20 2016

Fingerprint

Agaricus
Rotenone
Neuroblastoma
Antioxidants
Apoptosis
Membrane Potentials
Agaricales
Parkinson Disease
Cell Survival
Control Groups
Pharmaceutical Preparations
Proteins

Keywords

  • A. blazei
  • Apoptosis
  • Mitochondria membrane potential
  • Oxidative stress
  • Rotenone

ASJC Scopus subject areas

  • Neuroscience(all)
  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Venkatesh Gobi, V., Rajasankar, S., Ramkumar, M., Dhanalakshmi, C., Manivasagam, T., Justin Thenmozhi, A., ... Chidambaram, R. (Accepted/In press). Agaricus blazei extract attenuates rotenone-induced apoptosis through its mitochondrial protective and antioxidant properties in SH-SY5Y neuroblastoma cells. Nutritional Neuroscience, 1-11. https://doi.org/10.1080/1028415X.2016.1222332

Agaricus blazei extract attenuates rotenone-induced apoptosis through its mitochondrial protective and antioxidant properties in SH-SY5Y neuroblastoma cells. / Venkatesh Gobi, Veerappan; Rajasankar, Srinivasagam; Ramkumar, Muthu; Dhanalakshmi, Chinnasamy; Manivasagam, Thamilarasan; Justin Thenmozhi, Arokiasamy; Essa, Musthafa Mohamed; Chidambaram, Ranganathan.

In: Nutritional Neuroscience, 20.09.2016, p. 1-11.

Research output: Contribution to journalArticle

Venkatesh Gobi, Veerappan ; Rajasankar, Srinivasagam ; Ramkumar, Muthu ; Dhanalakshmi, Chinnasamy ; Manivasagam, Thamilarasan ; Justin Thenmozhi, Arokiasamy ; Essa, Musthafa Mohamed ; Chidambaram, Ranganathan. / Agaricus blazei extract attenuates rotenone-induced apoptosis through its mitochondrial protective and antioxidant properties in SH-SY5Y neuroblastoma cells. In: Nutritional Neuroscience. 2016 ; pp. 1-11.
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