Adopting the rapamycin trapping assay to track the trafficking of murine MHC class I alleles, H-2Kb

Esther Ghanem, Mohammed Al-Balushi

Research output: Contribution to journalArticle

Abstract

Background: In mammalian cells, the quality control (QC) of properly folded proteins is monitored in the early secretory pathway, particularly in the endoplasmic reticulum (ER). Several proteins, including our protein of interest, major histocompatibility complex class I (MHC class I), can bypass the first line of ER-QC and reside in post-ER compartments in an unfolded form. Such forms entail both monomeric and dimeric structures that are devoid of peptides and thus cannot fulfill the immunological function of antigen presentation at the cell surface. MHC class I structures become mature and properly folded once loaded with the appropriate peptides in the framework of the peptide loading complex (PLC). Despite the flood of information on the diverse trafficking behavior of different MHC class I alleles, there is still controversy on the actual trajectory followed by improperly folded murine MHC class I alleles, namely H-2Kb. In this study, we employ an in vitro rapamycin trapping assay, live cell imaging, and a biochemical COPII budding approach to further investigate the trafficking of H-2Kb beyond the level of the ER. Results: We confirm the egress of H-2Kb in an unfolded form to a post-ER compartment from where they can cycle back to the ER. Deciphering the exact identity of the post-ER compartment by laser scanning microscopy did not only point to the existence of the ERGIC and cis-Golgi compartments as residency areas for unfolded proteins, but also to the involvement of an addional compartment, that lies in close proximity and possesses high resemblance to the aforementioned compartments. Interestingly, we were capable of showing using the same rapamycin trapping assay that H-2Kb can undergo a potential maturation event during their cycling; this is attained upon addition of peptides and trapping of accumulated post-ER molecules at the cell surface. Conclusions: Our findings deepen the understanding of H-2Kb trafficking outside the ER and pave the way to decipher the role and the trafficking of certain PLC chaperones, such as tapasin, throughout H-2Kb post-ER QC. Finally, we demonstrate the plausible usage of the rapamycin assay to assess the trafficking of defected proteins especially in diseases and under therapeutic studies.

Original languageEnglish
Article number30
JournalBMC Cell Biology
Volume16
Issue number1
DOIs
Publication statusPublished - Dec 29 2015

Fingerprint

Sirolimus
Major Histocompatibility Complex
Endoplasmic Reticulum
Alleles
Peptides
Quality Control
Protein Unfolding
Proteins
Secretory Pathway
Antigen Presentation
Protein Transport
Internship and Residency
Confocal Microscopy

Keywords

  • (MHC) Major histocompatibility complex
  • (QC) Quality control
  • Intracellular trafficking
  • Protein sorting
  • Rapamycin trapping assay

ASJC Scopus subject areas

  • Cell Biology

Cite this

Adopting the rapamycin trapping assay to track the trafficking of murine MHC class I alleles, H-2Kb . / Ghanem, Esther; Al-Balushi, Mohammed.

In: BMC Cell Biology, Vol. 16, No. 1, 30, 29.12.2015.

Research output: Contribution to journalArticle

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