Adherence of Salmonella typhimurium to murine peritoneal macrophages is mediated by Lipopolysaccharide and complement receptors

Saif N. Al-Bahry, Thomas G. Pistole

Research output: Contribution to journalArticle

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Abstract

Adherence of Salmonella typhimurium to mouse peritoneal macrophages (M0) was monitored using a direct microscopic assay and flow cytometry. Competitive binding studies using wild type lipopolysaccharide and derivatives confirmed a role for this moiety in bacterial adherence. M0 pretreated with 2-deoxy-D-glucose exhibited lower binding activity than did untreated controls, suggesting involvement of either Fc or complement receptors. Preexposing M0 to Fc fragments, however, failed to reduce bacterial binding, thus eliminating a role for Fc receptors in this process. M0 pretreated with neutrophil elastase exhibited a diminished ability to bind S. typhimurium, suggesting involvement of complement receptor 1. Monoclonal antibodies M1/70 and M18/2, specific for epitopes on the α and β chains, respectively, of complement receptor 3, also blocked this adherence. In each case we were unable to eliminate completely bacterial adhesion to M0. Monoclonal antibodies to two additional M0 receptors, Mac-2 and Mac-3, did not block bacterial attachment. These data indicate that multiple mechanisms are involved in the initial adhesion of S. typhimurium to mouse M0.

Original languageEnglish
Pages (from-to)83-92
Number of pages10
JournalZentralblatt fur Bakteriologie
Volume286
Issue number1
Publication statusPublished - Jun 1997

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CD14 Antigens
Complement Receptors
Peritoneal Macrophages
Salmonella typhimurium
Fc Receptors
Macrophage-1 Antigen
Complement C1
Bacterial Adhesion
Immunoglobulin Fc Fragments
Leukocyte Elastase
Competitive Binding
Deoxyglucose
Lipopolysaccharides
Epitopes
Flow Cytometry
Monoclonal Antibodies

ASJC Scopus subject areas

  • Immunology

Cite this

Adherence of Salmonella typhimurium to murine peritoneal macrophages is mediated by Lipopolysaccharide and complement receptors. / Al-Bahry, Saif N.; Pistole, Thomas G.

In: Zentralblatt fur Bakteriologie, Vol. 286, No. 1, 06.1997, p. 83-92.

Research output: Contribution to journalArticle

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