Activity stabilization of Aspergillus niger and Escherichia coli phytases immobilized on allophanic synthetic compounds and montmorillonite nanoclays

Daniel Menezes-Blackburn, Milko Jorquera, Liliana Gianfreda, Maria Rao, Ralf Greiner, Elizabeth Garrido, María De la Luz Mora*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

65 Citations (Scopus)

Abstract

The aim of this work was to study the stabilization of the activity of two commercial microbial phytases (Aspergillus niger and Escherichia coli) after immobilization on nanoclays and to establish optimal conditions for their immobilization. Synthetic allophane, synthetic iron-coated allophanes and natural montmorillonite were chosen as solid supports for phytase immobilization. Phytase immobilization patterns at different pH values were strongly dependent on both enzyme and support characteristics. After immobilization, the residual activity of both phytases was higher under acidic conditions. Immobilization of phytases increased their thermal stability and improved resistance to proteolysis, particularly on iron-coated allophane (6% iron oxide), which showed activation energy (E(a)) and activation enthalpy (ΔH(#)) similar to free enzymes. Montmorillonite as well as allophanic synthetic compounds resulted in a good support for immobilization of E. coli phytase, but caused a severe reduction of A. niger phytase activity.

Original languageEnglish
Pages (from-to)9360-9367
Number of pages8
JournalBioresource Technology
Volume102
Issue number20
DOIs
Publication statusPublished - Oct 2011

Keywords

  • 6-Phytase/metabolism
  • Aluminum Silicates
  • Aspergillus niger/enzymology
  • Bentonite
  • Clay
  • Enzymes, Immobilized/metabolism
  • Escherichia coli/enzymology
  • Nanotechnology
  • Proteolysis
  • Temperature
  • Thermodynamics
  • X-Ray Diffraction

ASJC Scopus subject areas

  • Bioengineering
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment
  • Waste Management and Disposal

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