Accumulation of intermediate denitrifying compounds inhibiting biological denitrification on cathode in Microbial Fuel Cell

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Abstract

Background: Bio-cathode denitrifying microbial fuel cell (MFC) is a promising bio-electrochemical system (BES) where both the reactions of anodic oxidation and cathodic reduction are catalyzed by microorganisms. In this nitrogen removal process, a complete biological denitrification from nitrate (NO3 -) to molecular nitrogen (N2) was achieved by four reduction steps, forming nitrite (NO2 -), nitric oxide (NO) and nitrous oxide (N2O) as intermediate compounds. These enzymatic catalysis reductions are often slowed down on cathode electrode at the higher cathodic nitrate loading. This study investigated the cause for inhibition of the biological denitrification in a three-chambered MFC where the middle chamber acted as denitrifying bio-cathode and the two chambers at the side acted as bio-anode. Carbon fiber brushes were used as electrodes and nafion membranes were used as separator between the chambers. Results: The maximum power obtained was 14.63 W m-3 net cathodic compartment (NCC) (Rext =11.5γ) at an optimum nitrate loading of 0.15 kg NO3 --N m-3 NCC d-1. The accumulation of one of the intermediate denitrifying compound, e.g., NO2 - adversely affected biological denitrification rate on cathode. According to chemical kinetics, the accumulated NO2 - will form free nitrous acid (FNA, HNO2) in aqueous chemical system spontaneously. The study showed that approximately 45 % of the current production and 20 % of the total denitrification was decreased at a FNA concentration of 0.0014 ± 0.0001 mg HNO2 - N L-1 with an equivalent nitrite concentration of 6.2 ± 0.9 mg NO2 - - N L-1. Conclusions: The novel biological process indicates the potential of using denitrifying bio-cathode MFC for green energy production.

Original languageEnglish
JournalJournal of Environmental Health Science and Engineering
DOIs
Publication statusAccepted/In press - Nov 24 2015

Fingerprint

Bioelectric Energy Sources
Denitrification
Microbial fuel cells
fuel cell
denitrification
Electrodes
Cathodes
nitrate
Nitrates
nitrite
electrode
nitrogen
catalysis
nitric oxide
Nitrites
nitrous oxide
Nitrogen removal
biological processes
Nitric oxide
Anodic oxidation

Keywords

  • Bio-cathode
  • Biological denitrification
  • Bioremediation
  • Microbial fuel cell
  • Process inhibition

ASJC Scopus subject areas

  • Environmental Engineering
  • Health, Toxicology and Mutagenesis
  • Waste Management and Disposal
  • Water Science and Technology
  • Public Health, Environmental and Occupational Health
  • Pollution
  • Applied Microbiology and Biotechnology

Cite this

@article{6c793a7e14b147e69e13b29a2e0e1581,
title = "Accumulation of intermediate denitrifying compounds inhibiting biological denitrification on cathode in Microbial Fuel Cell",
abstract = "Background: Bio-cathode denitrifying microbial fuel cell (MFC) is a promising bio-electrochemical system (BES) where both the reactions of anodic oxidation and cathodic reduction are catalyzed by microorganisms. In this nitrogen removal process, a complete biological denitrification from nitrate (NO3 -) to molecular nitrogen (N2) was achieved by four reduction steps, forming nitrite (NO2 -), nitric oxide (NO) and nitrous oxide (N2O) as intermediate compounds. These enzymatic catalysis reductions are often slowed down on cathode electrode at the higher cathodic nitrate loading. This study investigated the cause for inhibition of the biological denitrification in a three-chambered MFC where the middle chamber acted as denitrifying bio-cathode and the two chambers at the side acted as bio-anode. Carbon fiber brushes were used as electrodes and nafion membranes were used as separator between the chambers. Results: The maximum power obtained was 14.63 W m-3 net cathodic compartment (NCC) (Rext =11.5γ) at an optimum nitrate loading of 0.15 kg NO3 --N m-3 NCC d-1. The accumulation of one of the intermediate denitrifying compound, e.g., NO2 - adversely affected biological denitrification rate on cathode. According to chemical kinetics, the accumulated NO2 - will form free nitrous acid (FNA, HNO2) in aqueous chemical system spontaneously. The study showed that approximately 45 {\%} of the current production and 20 {\%} of the total denitrification was decreased at a FNA concentration of 0.0014 ± 0.0001 mg HNO2 - N L-1 with an equivalent nitrite concentration of 6.2 ± 0.9 mg NO2 - - N L-1. Conclusions: The novel biological process indicates the potential of using denitrifying bio-cathode MFC for green energy production.",
keywords = "Bio-cathode, Biological denitrification, Bioremediation, Microbial fuel cell, Process inhibition",
author = "Abdullah Al-Mamun and Baawain, {Mahad Said}",
year = "2015",
month = "11",
day = "24",
doi = "10.1186/s40201-015-0236-5",
language = "English",
journal = "Journal of Environmental Health Science and Engineering",
issn = "2052-336X",
publisher = "Springer International Publishing AG",

}

TY - JOUR

T1 - Accumulation of intermediate denitrifying compounds inhibiting biological denitrification on cathode in Microbial Fuel Cell

AU - Al-Mamun, Abdullah

AU - Baawain, Mahad Said

PY - 2015/11/24

Y1 - 2015/11/24

N2 - Background: Bio-cathode denitrifying microbial fuel cell (MFC) is a promising bio-electrochemical system (BES) where both the reactions of anodic oxidation and cathodic reduction are catalyzed by microorganisms. In this nitrogen removal process, a complete biological denitrification from nitrate (NO3 -) to molecular nitrogen (N2) was achieved by four reduction steps, forming nitrite (NO2 -), nitric oxide (NO) and nitrous oxide (N2O) as intermediate compounds. These enzymatic catalysis reductions are often slowed down on cathode electrode at the higher cathodic nitrate loading. This study investigated the cause for inhibition of the biological denitrification in a three-chambered MFC where the middle chamber acted as denitrifying bio-cathode and the two chambers at the side acted as bio-anode. Carbon fiber brushes were used as electrodes and nafion membranes were used as separator between the chambers. Results: The maximum power obtained was 14.63 W m-3 net cathodic compartment (NCC) (Rext =11.5γ) at an optimum nitrate loading of 0.15 kg NO3 --N m-3 NCC d-1. The accumulation of one of the intermediate denitrifying compound, e.g., NO2 - adversely affected biological denitrification rate on cathode. According to chemical kinetics, the accumulated NO2 - will form free nitrous acid (FNA, HNO2) in aqueous chemical system spontaneously. The study showed that approximately 45 % of the current production and 20 % of the total denitrification was decreased at a FNA concentration of 0.0014 ± 0.0001 mg HNO2 - N L-1 with an equivalent nitrite concentration of 6.2 ± 0.9 mg NO2 - - N L-1. Conclusions: The novel biological process indicates the potential of using denitrifying bio-cathode MFC for green energy production.

AB - Background: Bio-cathode denitrifying microbial fuel cell (MFC) is a promising bio-electrochemical system (BES) where both the reactions of anodic oxidation and cathodic reduction are catalyzed by microorganisms. In this nitrogen removal process, a complete biological denitrification from nitrate (NO3 -) to molecular nitrogen (N2) was achieved by four reduction steps, forming nitrite (NO2 -), nitric oxide (NO) and nitrous oxide (N2O) as intermediate compounds. These enzymatic catalysis reductions are often slowed down on cathode electrode at the higher cathodic nitrate loading. This study investigated the cause for inhibition of the biological denitrification in a three-chambered MFC where the middle chamber acted as denitrifying bio-cathode and the two chambers at the side acted as bio-anode. Carbon fiber brushes were used as electrodes and nafion membranes were used as separator between the chambers. Results: The maximum power obtained was 14.63 W m-3 net cathodic compartment (NCC) (Rext =11.5γ) at an optimum nitrate loading of 0.15 kg NO3 --N m-3 NCC d-1. The accumulation of one of the intermediate denitrifying compound, e.g., NO2 - adversely affected biological denitrification rate on cathode. According to chemical kinetics, the accumulated NO2 - will form free nitrous acid (FNA, HNO2) in aqueous chemical system spontaneously. The study showed that approximately 45 % of the current production and 20 % of the total denitrification was decreased at a FNA concentration of 0.0014 ± 0.0001 mg HNO2 - N L-1 with an equivalent nitrite concentration of 6.2 ± 0.9 mg NO2 - - N L-1. Conclusions: The novel biological process indicates the potential of using denitrifying bio-cathode MFC for green energy production.

KW - Bio-cathode

KW - Biological denitrification

KW - Bioremediation

KW - Microbial fuel cell

KW - Process inhibition

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U2 - 10.1186/s40201-015-0236-5

DO - 10.1186/s40201-015-0236-5

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