Abundance and diversity of aerobic heterotrophic microorganisms and their interaction with cyanobacteria in the oxic layer of an intertidal hypersaline cyanobacterial mat

Raeid Abed, Katharina Kohls, Julie Leloup, Dirk de Beer

Research output: Contribution to journalArticle

Abstract

Aerobic heterotrophic microorganisms (AH) play a significant role in carbon cycling in cyanobacterial mats; however, little is known about their abundance, diversity and interaction with cyanobacteria. Using catalyzed reporter deposition fluorescence in situ hybridization (CARD-FISH), bacterial counts in the mat's oxic layer reached a mean of 2.23 ± 0.4 × 1010 cells g-1. Cultivation of AH yielded strains belonging to Actinobacteria, Bacteroidetes, Firmicutes, Gammaproteobacteria and Haloarchaea. 16S rRNA bacterial sequences retrieved from the mat's oxic layer were related to Bacteroidetes, Chloroflexi and Proteobacteria, whereas archaeal sequences belonged to Crenarchaeota and Haloarchaea. Monocultures of cyanobacteria from the same mat were associated with different AH, although Bacteroidetes were found in most cultures. CARD-FISH showed that Bacteroidetes- and Chloroflexi-related bacteria were closely associated with filaments of Microcoleus chthonoplastes. The growth of an axenic culture of M. chthonoplastes PCC7420 was stimulated on the addition of a filtrate obtained from a non-axenic Microcoleus culture and containing only AH and released substances. In contrast, a similar filtrate from a non-axenic Cyanothece-related culture killed Cyanothece PCC 7418. We conclude that a diverse community of AH exist in close association with cyanobacteria in microbial mats and the interactions between AH and cyanobacteria are species-specific and involve the release of substances.

Original languageEnglish
JournalFEMS Microbiology Ecology
Volume94
Issue number2
DOIs
Publication statusPublished - Feb 1 2018

Fingerprint

Bacteroidetes
Cyanobacteria
Cyanothece
cyanobacterium
microorganism
Chloroflexi
Fluorescence In Situ Hybridization
Crenarchaeota
Microbial Interactions
Axenic Culture
Gammaproteobacteria
Proteobacteria
Bacterial Load
Actinobacteria
fluorescence
Carbon
microbial mat
monoculture
Bacteria
Growth

Keywords

  • aerobic heterotrophs
  • archaea
  • CARD-FISH
  • Cyanobacteria
  • cyanobacterial mats
  • qPCR

ASJC Scopus subject areas

  • Microbiology
  • Ecology
  • Applied Microbiology and Biotechnology

Cite this

Abundance and diversity of aerobic heterotrophic microorganisms and their interaction with cyanobacteria in the oxic layer of an intertidal hypersaline cyanobacterial mat. / Abed, Raeid; Kohls, Katharina; Leloup, Julie; de Beer, Dirk.

In: FEMS Microbiology Ecology, Vol. 94, No. 2, 01.02.2018.

Research output: Contribution to journalArticle

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