A pilot study of HTLV-I infection in high-risk individuals & their family members from India

S. Ramalingam, R. Kannangai, K. J. Prakash, K. Ajithkumar, M. Jacob, R. George, S. Pradeepkumar, D. Daniel, D. Dennison, P. G. Babu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background & objectives: Human T lymphotropic virus-I (HTLV-I) has been associated with adult T cell lymphoma/leukemia (ATLL). There are Indian studies on HTLV-I infection among people with sexually transmitted infection, but no large study has been conducted on individuals with haematological malignancies. In this group of individuals, serology is known to under-diagnose HTLV-I infection. This study was carried out to identify serologically and where possible with molecular techniques, HTLV-I infection in individuals with haematological malignancies. To understand the modes of transmission, family members of individuals with provein HTLV-I infection were also studied. Individuals with sexually transmitted infection (STI), blood donors and pregnant women were also studied. Methods: Particle agglutination test was used to detect antibody to HTLV-I. HTLV genome was amplified by polymerase chain reaction (PCR) and detected with probes by digoxiginin (Dig) ELISA. Results: There was no serological evidence of HTLV-I infection among the healthy blood donors and pregnant women studied. High prevalence of anti-HTLV-I antibody was identified in the patients with haematological malignancies (8 of 86 patients, 9.3%) and a lower prevalence in individuals with STI (8 of 670 individuals, 1.2%). In the STI group, all 8 individuals seroreactive to HTLV-I were coinfected with human immunodeficiency virus (HIV). In the group with haematological malignancies, three of 22 (13.6%) patients with leukemia, 3 of 11 (27.3%) with cutaneous T-cell lymphoma (CTCL) and 2 of 53 (3.8%) with lymphoma were reactive for anti-HTLV-1 antibody. In this group, PCR identified all the seroreactive individuals tested. There were also seronegative infected individuals who were only identified by PCR. There was also a large number of seronegative family members who were only positive by PCR. Interpretation & conclusion: The study revealed a strong disease association of HTLV-I with haematological malignancies and evidence for both horizontal and vertical transmission of the infection in the Indian population. HTLV-I infection appears to be common among family members of individuals with HTLV-I associated haematological malignancies.

Original languageEnglish
Pages (from-to)201-209
Number of pages9
JournalIndian Journal of Medical Research
Volume113
Issue numberJUN.
Publication statusPublished - 2001

Fingerprint

Virus Diseases
Viruses
India
Hematologic Neoplasms
Sexually Transmitted Diseases
Polymerase chain reaction
Polymerase Chain Reaction
T-cells
Blood Donors
Pregnant Women
Deltaretrovirus Antibodies
Antibodies
HTLV-I Antibodies
Blood
Cutaneous T-Cell Lymphoma
Adult T Cell Leukemia Lymphoma
Agglutination Tests
Human T-lymphotropic virus 1
Infectious Disease Transmission
Serology

Keywords

  • Cutaneous T cell lymphoma
  • Family
  • HTLV-I
  • India
  • Leukemia
  • Lymphoma

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Ramalingam, S., Kannangai, R., Prakash, K. J., Ajithkumar, K., Jacob, M., George, R., ... Babu, P. G. (2001). A pilot study of HTLV-I infection in high-risk individuals & their family members from India. Indian Journal of Medical Research, 113(JUN.), 201-209.

A pilot study of HTLV-I infection in high-risk individuals & their family members from India. / Ramalingam, S.; Kannangai, R.; Prakash, K. J.; Ajithkumar, K.; Jacob, M.; George, R.; Pradeepkumar, S.; Daniel, D.; Dennison, D.; Babu, P. G.

In: Indian Journal of Medical Research, Vol. 113, No. JUN., 2001, p. 201-209.

Research output: Contribution to journalArticle

Ramalingam, S, Kannangai, R, Prakash, KJ, Ajithkumar, K, Jacob, M, George, R, Pradeepkumar, S, Daniel, D, Dennison, D & Babu, PG 2001, 'A pilot study of HTLV-I infection in high-risk individuals & their family members from India', Indian Journal of Medical Research, vol. 113, no. JUN., pp. 201-209.
Ramalingam S, Kannangai R, Prakash KJ, Ajithkumar K, Jacob M, George R et al. A pilot study of HTLV-I infection in high-risk individuals & their family members from India. Indian Journal of Medical Research. 2001;113(JUN.):201-209.
Ramalingam, S. ; Kannangai, R. ; Prakash, K. J. ; Ajithkumar, K. ; Jacob, M. ; George, R. ; Pradeepkumar, S. ; Daniel, D. ; Dennison, D. ; Babu, P. G. / A pilot study of HTLV-I infection in high-risk individuals & their family members from India. In: Indian Journal of Medical Research. 2001 ; Vol. 113, No. JUN. pp. 201-209.
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abstract = "Background & objectives: Human T lymphotropic virus-I (HTLV-I) has been associated with adult T cell lymphoma/leukemia (ATLL). There are Indian studies on HTLV-I infection among people with sexually transmitted infection, but no large study has been conducted on individuals with haematological malignancies. In this group of individuals, serology is known to under-diagnose HTLV-I infection. This study was carried out to identify serologically and where possible with molecular techniques, HTLV-I infection in individuals with haematological malignancies. To understand the modes of transmission, family members of individuals with provein HTLV-I infection were also studied. Individuals with sexually transmitted infection (STI), blood donors and pregnant women were also studied. Methods: Particle agglutination test was used to detect antibody to HTLV-I. HTLV genome was amplified by polymerase chain reaction (PCR) and detected with probes by digoxiginin (Dig) ELISA. Results: There was no serological evidence of HTLV-I infection among the healthy blood donors and pregnant women studied. High prevalence of anti-HTLV-I antibody was identified in the patients with haematological malignancies (8 of 86 patients, 9.3{\%}) and a lower prevalence in individuals with STI (8 of 670 individuals, 1.2{\%}). In the STI group, all 8 individuals seroreactive to HTLV-I were coinfected with human immunodeficiency virus (HIV). In the group with haematological malignancies, three of 22 (13.6{\%}) patients with leukemia, 3 of 11 (27.3{\%}) with cutaneous T-cell lymphoma (CTCL) and 2 of 53 (3.8{\%}) with lymphoma were reactive for anti-HTLV-1 antibody. In this group, PCR identified all the seroreactive individuals tested. There were also seronegative infected individuals who were only identified by PCR. There was also a large number of seronegative family members who were only positive by PCR. Interpretation & conclusion: The study revealed a strong disease association of HTLV-I with haematological malignancies and evidence for both horizontal and vertical transmission of the infection in the Indian population. HTLV-I infection appears to be common among family members of individuals with HTLV-I associated haematological malignancies.",
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