A novel phosphorus biofertilization strategy using cattle manure treated with phytase-nanoclay complexes

Daniel Menezes-Blackburn, Milko A. Jorquera, Liliana Gianfreda, Ralf Greiner, María de la Luz Mora*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)


The aim of this work was to evaluate the treatment of cattle manure with phytases stabilized in allophanic nanoclays as a potential novel phosphorus (P) biofertilization technology for crops grown in volcanic soils (Andisol). Furthermore, because the optimal pH for commercial phytase catalysis does not match the natural pH of manure, a complementary experiment was set up to evaluate the effect of manure inoculation with an alkaline phytase-producing bacterium. Finally, phytase-treated soil, manure, and soil-manure mixtures were evaluated for their P-supplying capacity to wheat plants grown under greenhouse conditions. Treating cattle manure with phytases stabilized in nanoclays resulted in a significant (P ≤ 0.05) increase of inorganic P in soil extracts (NaOH-EDTA and Olsen). The use of phytase-treated cattle manure increased dry weights by 10 % and the P concentration by 39 % in wheat plants grown under greenhouse conditions, which is equivalent to a P fertilizer rate of about 150 kg of P per hectare. The inoculation of cattle manure with β-propeller phytase-producing bacteria led to an ~10 % increase in inorganic P in the manure extracts. However, applying inoculated manure to soil did not significantly increase wheat yield or P acquisition responses. Our results suggest that the novel approach of incubating cattle manure with phytases stabilized in nanoclay enhances the organic P cycling and P nutrition of plants grown in P-deficient soils.

Original languageEnglish
Pages (from-to)583-592
Number of pages10
JournalBiology and Fertility of Soils
Issue number4
Publication statusPublished - 2014


  • Biofertilization
  • Cattle manure
  • Organic phosphorus
  • Phosphorus
  • Phytase
  • Phytate
  • Volcanic soils
  • Wheat

ASJC Scopus subject areas

  • Microbiology
  • Agronomy and Crop Science
  • Soil Science

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