A novel model using mean platelet volume and neutrophil to lymphocyte ratio as a marker of nonalcoholic steatohepatitis in NAFLD patients: multicentric study

Ahmed Abdel-Razik, Nasser Mousa, Walaa Shabana, Mohamed Refaey, Youssif ElMahdy, Rania Elhelaly, Rasha Elzehery, Khaled Zalata, Mohammad Arafa, Sherif Elbaz, Mohamed Hafez, Mahmoud Awad

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BACKGROUND AND AIM: Nonalcoholic fatty liver disease (NAFLD) is a leading cause of progressive and chronic liver injury. Mean platelet volume (MPV) and the neutrophil-lymphocyte ratio (N/L ratio) may be considered cheap and simple markers of inflammation in many disorders. We aimed to investigate the clinical utility of MPV and the N/L ratio to predict fibrosis in NAFLD patients and the presence of nonalcoholic steatohepatitis (NASH).

MATERIALS AND METHODS: A total of 873 patients with biopsy-proven NAFLD and 150 healthy controls were included. Patients were divided into two groups: non-NASH group (n=753) and NASH group (n=120). Liver biopsy, MPV, lymphocyte, and neutrophil counts were registered; the N/L ratio was calculated. Proinflammatory cytokines (tumor necrosis factor-α and interleukin-6) were measured by an ELISA.

RESULTS: NASH patients had higher MPV compared with non-NASH patients (10.9±1.8 and 9.5±1.6 fl, respectively, P<0.001). MPV correlated positively with the NAFLD activity score, proinflammatory cytokines, and C-reactive protein (CRP) (P<0.001). Patients with advanced fibrosis (F3-4) had increased MPV (11.3±0.9 fl) compared with patients with early fibrosis (F1-2) (10.2±0.8 fl, P<0.001). NASH patients had an increased N/L ratio compared with non-NASH cases (2.6±1.1 and 1.9±0.7 fl, respectively, P<0.001). The N/L ratio correlated positively with NAFLD activity score, proinflammatory cytokines, and CRP (P<0.001). In addition, patients with advanced fibrosis (F3-4) had an N/L ratio (2.5±1.1) comparable with that of patients with early fibrosis (F1-2) (1.8±0.9) (P<0.001).

CONCLUSION: MPV and the N/L ratio were elevated in NASH patients versus non-NASH cases, and in patients with advanced fibrosis (F3-4) versus early fibrosis (F1-2). They can be used as noninvasive novel markers to predict advanced disease.

Original languageEnglish
Pages (from-to)e1-9
JournalEuropean Journal of Gastroenterology and Hepatology
Issue number1
Publication statusPublished - Jan 2016


  • Adult
  • Alanine Transaminase/blood
  • Area Under Curve
  • Biomarkers/blood
  • C-Reactive Protein/metabolism
  • Case-Control Studies
  • Female
  • Humans
  • Interleukin-6/blood
  • Liver Cirrhosis/blood
  • Logistic Models
  • Lymphocyte Count
  • Male
  • Mean Platelet Volume
  • Middle Aged
  • Neutrophils
  • Non-alcoholic Fatty Liver Disease/blood
  • Platelet Count
  • Predictive Value of Tests
  • Prospective Studies
  • ROC Curve
  • Tumor Necrosis Factor-alpha/blood

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