A novel GlcNAcα1-HPO3-6Gal(1-1)ceramide antigen and alkylated inositol-phosphoglycerolipids expressed by the liver fluke Fasciola hepatica

Manfred Wuhrer, Christiane Grimm, Ulrich Zähringer, Roger D. Dennis, Clemens M. Berkefeld, Mohamed A. Idris, Rudolf Geyer

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23 Citations (Scopus)

Abstract

The acidic (glyco)lipids of the parasitic liver fluke Fasciola hepatica exhibited two different phosphate-containing species, designated AL-I and AL-II, which were analyzed by MALDI-TOF MS, ESI MS, NMR, methylation analysis, and combined GC-MS in conjunction with HF treatment. AL-I was structurally determined as 1-O-hexadecyl-sn-glycerol-3-phosphoinositol, an ether bond variant of lysophosphatidylinositol. The structure of AL-II was shown to be GlcNAcα1-HPO3-6Gal(1-1)ceramide. Ceramide analysis revealed as major components 2-hydroxyoctadecanoic acid [18:0(2-OH)] together with C18- and C20-phytosphingosines. AL-II was apparently highly antigenic and strongly recognized by both animal- and human-F. hepatica infection sera. Furthermore, inhibition ELISAs revealed that the unusual antigenic determinant GlcNAcα1-HPO3- phosphate might have a potential in the serodiagnosis of F. hepatica infections.

Original languageEnglish
Pages (from-to)129-137
Number of pages9
JournalGlycobiology
Volume13
Issue number2
DOIs
Publication statusPublished - Feb 1 2003

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Keywords

  • Electrospray mass spectrometry
  • Liver fluke glycolipids
  • Oligosaccharide structural analysis
  • Parasitic trematode

ASJC Scopus subject areas

  • Biochemistry

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