TY - JOUR
T1 - A novel GlcNAcα1-HPO3-6Gal(1-1)ceramide antigen and alkylated inositol-phosphoglycerolipids expressed by the liver fluke Fasciola hepatica
AU - Wuhrer, Manfred
AU - Grimm, Christiane
AU - Zähringer, Ulrich
AU - Dennis, Roger D.
AU - Berkefeld, Clemens M.
AU - Idris, Mohamed A.
AU - Geyer, Rudolf
PY - 2003/2/1
Y1 - 2003/2/1
N2 - The acidic (glyco)lipids of the parasitic liver fluke Fasciola hepatica exhibited two different phosphate-containing species, designated AL-I and AL-II, which were analyzed by MALDI-TOF MS, ESI MS, NMR, methylation analysis, and combined GC-MS in conjunction with HF treatment. AL-I was structurally determined as 1-O-hexadecyl-sn-glycerol-3-phosphoinositol, an ether bond variant of lysophosphatidylinositol. The structure of AL-II was shown to be GlcNAcα1-HPO3-6Gal(1-1)ceramide. Ceramide analysis revealed as major components 2-hydroxyoctadecanoic acid [18:0(2-OH)] together with C18- and C20-phytosphingosines. AL-II was apparently highly antigenic and strongly recognized by both animal- and human-F. hepatica infection sera. Furthermore, inhibition ELISAs revealed that the unusual antigenic determinant GlcNAcα1-HPO3- phosphate might have a potential in the serodiagnosis of F. hepatica infections.
AB - The acidic (glyco)lipids of the parasitic liver fluke Fasciola hepatica exhibited two different phosphate-containing species, designated AL-I and AL-II, which were analyzed by MALDI-TOF MS, ESI MS, NMR, methylation analysis, and combined GC-MS in conjunction with HF treatment. AL-I was structurally determined as 1-O-hexadecyl-sn-glycerol-3-phosphoinositol, an ether bond variant of lysophosphatidylinositol. The structure of AL-II was shown to be GlcNAcα1-HPO3-6Gal(1-1)ceramide. Ceramide analysis revealed as major components 2-hydroxyoctadecanoic acid [18:0(2-OH)] together with C18- and C20-phytosphingosines. AL-II was apparently highly antigenic and strongly recognized by both animal- and human-F. hepatica infection sera. Furthermore, inhibition ELISAs revealed that the unusual antigenic determinant GlcNAcα1-HPO3- phosphate might have a potential in the serodiagnosis of F. hepatica infections.
KW - Electrospray mass spectrometry
KW - Liver fluke glycolipids
KW - Oligosaccharide structural analysis
KW - Parasitic trematode
UR - http://www.scopus.com/inward/record.url?scp=0037322008&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037322008&partnerID=8YFLogxK
U2 - 10.1093/glycob/cwg005
DO - 10.1093/glycob/cwg005
M3 - Article
C2 - 12626405
AN - SCOPUS:0037322008
VL - 13
SP - 129
EP - 137
JO - Glycobiology
JF - Glycobiology
SN - 0959-6658
IS - 2
ER -