A novel biosensor for detecting toxicity in water using sulfur-oxidizing bacteria

Sang Eun Oh; Sedky H.A. Hassan; Steven W. Van Ginkel

doi:10.1016/j.snb.2010.01.052

A novel biosensor for detecting toxicity in water using sulfur-oxidizing bacteria

Sang Eun Oh^*, Sedky H.A. Hassan, Steven W. Van Ginkel

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

57 Citations (Scopus)

Abstract

A novel toxicity detection methodology based on sulfur-oxidizing bacteria (SOB) has been developed for the rapid and reliable detection of toxic chemicals in water. The methodology exploits the ability of SOB to oxidize sulfur particles in the presence of oxygen to produce sulfuric acid according to the following equation: S + H₂O + 1.5O₂ → SO ₄^2- + 2H⁺, ΔG°′ = -587.1 kJ/reaction. The reaction results in an increase in electrical conductivity (EC) and a decrease in pH as SOB convert insoluble sulfur particles to sulfate and protons. The proposed technique is validated using EC and pH data. Using a synthetic stream water (EC = 0.12 mS/cm and pH 7.2), the baseline steady-state EC and pH values were ∼1.0 mS/cm and ∼2.5 over 30 days of testing when hexavalent chromium (Cr⁶⁺) was not added to the system. When Cr ⁶⁺ was added to the system, the effluent EC decreased and the pH increased due to inhibition of SOB. We found that the system can detect Cr ⁶⁺ at a concentration of 5 ppb which is lower than any method to date.

Original language	English
Pages (from-to)	17-21
Number of pages	5
Journal	Sensors and Actuators, B: Chemical
Volume	154
Issue number	1
DOIs	https://doi.org/10.1016/j.snb.2010.01.052
Publication status	Published - May 20 2011
Externally published	Yes

Keywords

Biosensor
Electrical conductivity
Sulfur particles
Sulfur-oxidizing bacteria
Toxicity

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.snb.2010.01.052

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title = "A novel biosensor for detecting toxicity in water using sulfur-oxidizing bacteria",

abstract = "A novel toxicity detection methodology based on sulfur-oxidizing bacteria (SOB) has been developed for the rapid and reliable detection of toxic chemicals in water. The methodology exploits the ability of SOB to oxidize sulfur particles in the presence of oxygen to produce sulfuric acid according to the following equation: S + H2O + 1.5O2 → SO 42- + 2H+, ΔG°′ = -587.1 kJ/reaction. The reaction results in an increase in electrical conductivity (EC) and a decrease in pH as SOB convert insoluble sulfur particles to sulfate and protons. The proposed technique is validated using EC and pH data. Using a synthetic stream water (EC = 0.12 mS/cm and pH 7.2), the baseline steady-state EC and pH values were ∼1.0 mS/cm and ∼2.5 over 30 days of testing when hexavalent chromium (Cr6+) was not added to the system. When Cr 6+ was added to the system, the effluent EC decreased and the pH increased due to inhibition of SOB. We found that the system can detect Cr 6+ at a concentration of 5 ppb which is lower than any method to date.",

keywords = "Biosensor, Electrical conductivity, Sulfur particles, Sulfur-oxidizing bacteria, Toxicity",

author = "Oh, {Sang Eun} and Hassan, {Sedky H.A.} and {Van Ginkel}, {Steven W.}",

note = "Funding Information: This work was supported by the Eco-star project funded by the Korean Government and a grant from the Institute of Environmental Research at Kangwon National University. ",

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AU - Oh, Sang Eun

AU - Hassan, Sedky H.A.

AU - Van Ginkel, Steven W.

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PY - 2011/5/20

Y1 - 2011/5/20

N2 - A novel toxicity detection methodology based on sulfur-oxidizing bacteria (SOB) has been developed for the rapid and reliable detection of toxic chemicals in water. The methodology exploits the ability of SOB to oxidize sulfur particles in the presence of oxygen to produce sulfuric acid according to the following equation: S + H2O + 1.5O2 → SO 42- + 2H+, ΔG°′ = -587.1 kJ/reaction. The reaction results in an increase in electrical conductivity (EC) and a decrease in pH as SOB convert insoluble sulfur particles to sulfate and protons. The proposed technique is validated using EC and pH data. Using a synthetic stream water (EC = 0.12 mS/cm and pH 7.2), the baseline steady-state EC and pH values were ∼1.0 mS/cm and ∼2.5 over 30 days of testing when hexavalent chromium (Cr6+) was not added to the system. When Cr 6+ was added to the system, the effluent EC decreased and the pH increased due to inhibition of SOB. We found that the system can detect Cr 6+ at a concentration of 5 ppb which is lower than any method to date.

AB - A novel toxicity detection methodology based on sulfur-oxidizing bacteria (SOB) has been developed for the rapid and reliable detection of toxic chemicals in water. The methodology exploits the ability of SOB to oxidize sulfur particles in the presence of oxygen to produce sulfuric acid according to the following equation: S + H2O + 1.5O2 → SO 42- + 2H+, ΔG°′ = -587.1 kJ/reaction. The reaction results in an increase in electrical conductivity (EC) and a decrease in pH as SOB convert insoluble sulfur particles to sulfate and protons. The proposed technique is validated using EC and pH data. Using a synthetic stream water (EC = 0.12 mS/cm and pH 7.2), the baseline steady-state EC and pH values were ∼1.0 mS/cm and ∼2.5 over 30 days of testing when hexavalent chromium (Cr6+) was not added to the system. When Cr 6+ was added to the system, the effluent EC decreased and the pH increased due to inhibition of SOB. We found that the system can detect Cr 6+ at a concentration of 5 ppb which is lower than any method to date.

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KW - Toxicity

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A novel biosensor for detecting toxicity in water using sulfur-oxidizing bacteria

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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