A new phytoplasma associated with witches' broom of Cassia italica in Oman

Akhtar Jamal Khan, Ali Masoud Al-Subhi, Alberto Calari*, Nadyla Abubaker Al-Saady, Assunta Bertaccini

*Corresponding author for this work

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In several Oman locations plants of Cassia itálica exhibit witches' broom symptoms. Samples were collected from four locations, and examined for phytoplasma presence. PCR amplification using ribosomal phytoplasma primers followed by RFLP analyses indicates that the phytoplasmas present in these samples were undistinguishable from each other, but differed from those reported in the literature. RFLP and phylogenetic analysis of 16S rRNA gene plus spacer sequence confirmed that the closest phytoplasma relatives were members of the pigeon pea witches' broom phytoplasma ribosomal group (16SrIX), sharing a 93-97% sequence similarity. Nested PCR experiments using primers amplifying the gene coding for ribosomal protein S3 provided amplification from phytoplasmas detected in C italica, after sequencing 600 bp in this gene the higher homology showed was 78% with phytoplasmas related to 'Candidatus Phytoplasma phoenicium' and 77% with phytoplasmas belonging to ribosomal group 16SrIX.

Original languageEnglish
Pages (from-to)269-270
Number of pages2
JournalBulletin of Insectology
Volume60
Issue number2
Publication statusPublished - 2007

Keywords

  • PCR/RFLP analyses
  • Phytoplasma
  • Plant disease
  • Ribosomal gene
  • Ribosomal protein s3 gene
  • Sequencing

ASJC Scopus subject areas

  • Insect Science

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    Khan, A. J., Al-Subhi, A. M., Calari, A., Al-Saady, N. A., & Bertaccini, A. (2007). A new phytoplasma associated with witches' broom of Cassia italica in Oman. Bulletin of Insectology, 60(2), 269-270.