A High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in Horses

Cécile Beck, Philippe Desprès, Sylvie Paulous, Jessica Vanhomwegen, Steeve Lowenski, Norbert Nowotny, Benoit Durand, Annabelle Garnier, Sandra Blaise-Boisseau, Edouard Guitton, Takashi Yamanaka, Stéphan Zientara, Sylvie Lecollinet

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

West Nile virus (WNV), Japanese encephalitis virus (JEV), and tick-borne encephalitis virus (TBEV) are flaviviruses responsible for severe neuroinvasive infections in humans and horses. The confirmation of flavivirus infections is mostly based on rapid serological tests such as enzyme-linked immunosorbent assays (ELISAs). These tests suffer from poor specificity, mainly due to antigenic cross-reactivity among flavivirus members. Robust diagnosis therefore needs to be validated through virus neutralisation tests (VNTs) which are time-consuming and require BSL3 facilities. The flavivirus envelope (E) glycoprotein ectodomain is composed of three domains (D) named DI, DII, and DIII, with EDIII containing virus-specific epitopes. In order to improve the serological differentiation of flavivirus infections, the recombinant soluble ectodomain of WNV E (WNV.sE) and EDIIIs (rEDIIIs) of WNV, JEV, and TBEV were synthesised using the Drosophila S2 expression system. Purified antigens were covalently bonded to fluorescent beads. The microspheres coupled to WNV.sE or rEDIIIs were assayed with about 300 equine immune sera from natural and experimental flavivirus infections and 172 nonimmune equine sera as negative controls. rEDIII-coupled microspheres captured specific antibodies against WNV, TBEV, or JEV in positive horse sera. This innovative multiplex immunoassay is a powerful alternative to ELISAs and VNTs for veterinary diagnosis of flavivirus-related diseases.

Original languageEnglish
Article number678084
JournalBioMed Research International
Volume2015
DOIs
Publication statusPublished - 2015

Fingerprint

Horse Diseases
Flavivirus
West Nile virus
Serologic Tests
Viruses
Immunoassay
Japanese Encephalitis Virus
Flavivirus Infections
Tick-Borne Encephalitis Viruses
Horses
Neutralization Tests
Microspheres
Enzyme-Linked Immunosorbent Assay
Serum
Immunosorbents
Drosophila
Immune Sera
Epitopes
Assays
Antigens

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

A High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in Horses. / Beck, Cécile; Desprès, Philippe; Paulous, Sylvie; Vanhomwegen, Jessica; Lowenski, Steeve; Nowotny, Norbert; Durand, Benoit; Garnier, Annabelle; Blaise-Boisseau, Sandra; Guitton, Edouard; Yamanaka, Takashi; Zientara, Stéphan; Lecollinet, Sylvie.

In: BioMed Research International, Vol. 2015, 678084, 2015.

Research output: Contribution to journalArticle

Beck, C, Desprès, P, Paulous, S, Vanhomwegen, J, Lowenski, S, Nowotny, N, Durand, B, Garnier, A, Blaise-Boisseau, S, Guitton, E, Yamanaka, T, Zientara, S & Lecollinet, S 2015, 'A High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in Horses', BioMed Research International, vol. 2015, 678084. https://doi.org/10.1155/2015/678084
Beck, Cécile ; Desprès, Philippe ; Paulous, Sylvie ; Vanhomwegen, Jessica ; Lowenski, Steeve ; Nowotny, Norbert ; Durand, Benoit ; Garnier, Annabelle ; Blaise-Boisseau, Sandra ; Guitton, Edouard ; Yamanaka, Takashi ; Zientara, Stéphan ; Lecollinet, Sylvie. / A High-Performance Multiplex Immunoassay for Serodiagnosis of Flavivirus-Associated Neurological Diseases in Horses. In: BioMed Research International. 2015 ; Vol. 2015.
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