A handheld 3D-printed microchip for simple integration of the H2O2-producing enzymatic reactions with subsequent chemiluminescence detection: Application for sugars

Haider A.J. Al Lawati*, Javad Hassanzadeh, Nafiseh Bagheri

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Herein, a novel lab-on-a-chip (LoC) device fabricated by 3D printing based on H2O2-producing enzymatic reactions with sensitive chemiluminescence (CL) detection was developed to measure different sugars, including glucose, fructose, sucrose, and maltose, in honey, juice, and rice flour samples. The pumpless microchip included two main parts, separated by new cone-shape blocking valves; part A for sample introduction and subsequent enzymatic reaction, besides the CL reagent (luminol) container, and part B for detection. The specific enzyme(s) were embedded into the pores of the zinc zeolite-imidazole framework (ZIF-8) to improve their storage stability. By opening the valves, H2O2 produced by enzymatic reaction and luminol could flow through the designed channels into the detection zone on part B, where a 2D cobalt-imidazole framework was embedded to improve the luminol-H2O2 CL emission. The obtained signal was proportional to the considered sugar concentration, with the detection limits range of 20–268 µM.

Original languageEnglish
Article number132469
JournalFood Chemistry
Volume383
DOIs
Publication statusPublished - Jul 30 2022
Externally publishedYes

Keywords

  • Chemiluminescence
  • Integrated microchip
  • Lab on a chip
  • Metal-organic frameworks
  • Sugars

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science

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