A divergent isolate of Tomato yellow leaf curl virus from Oman with an associated DNAβ satellite

An evolutionary link between Asian and the Middle Eastern virus-satellite complexes

Akhtar Jamal Khan, Ali M. Idris, Nadiya Abubaker Al-Saady, Madleen Said Al-Mahruki, Ali Masoud Al-Subhi, J. K. Brown

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Tomato is cultivated in the coastal region of Al-Batinah, in the Sultanate of Oman, during the winter season, to meet the high demand for fresh produce in the domestic market. In order to identify the causal agent of a widespread disease associated with infestations of the whitefly Bemisia tabaci (Genn.) leaves were collected from tomato plants showing symptoms characteristic of the disease in Al-Batinah during 2004 and 2005. Total nucleic acids were isolated from the tomato leaves and used as the template for Φ29 DNA polymerase amplification of begomoviral circular DNA. Putative full unit length begomoviral DNA multimers were digested with Nco I and cloned into the plasmid vector pGEM7Zf+. The complete nucleotide (nt) sequence was determined as 2,765 bases, indicative of a monopartite begomoviral genome. A comparison of the genome sequence for the seven field isolates examined, indicated that they shared 99% nt identity. The virus from Oman was most closely related to TYLCV-IR at 91% nt identity, a monopartite begomoviral species described previously from Iran. Based on the guidelines of the ICTV the Oman isolate has been designated TYLCV-Om and is considered an isolate of TYLCV-IR. A satellite DNA (satDNA β), was amplified by polymerase chain reaction using degenerate primers and cloned, and the DNA sequence was determined. Analysis of the complete nt sequence of 1,371 bases indicated that the satDNA shared 88.5% similarity with its closest relatives, which are DNAβ molecules from tomato in Pakistan. This is the first report of a satDNA β associated with the TYLCV species. The TYLCV-Om and associated satDNA, thus represent a begomovirus-complex at the Asian-Middle East crossroads that quiet uniquely share geographical and genetic hallmarks of both.

Original languageEnglish
Pages (from-to)169-176
Number of pages8
JournalVirus Genes
Volume36
Issue number1
DOIs
Publication statusPublished - Feb 2008

Fingerprint

Oman
Satellite DNA
Satellite Viruses
Lycopersicon esculentum
Viruses
Nucleotides
Begomovirus
Genome
Circular DNA
Hemiptera
Middle East
DNA
Pakistan
DNA-Directed DNA Polymerase
Iran
Nucleic Acids
Plasmids
Guidelines
Polymerase Chain Reaction

Keywords

  • Begomovirus
  • Bridge species
  • DNA satellite
  • Evolution
  • Geminiviridae
  • Tomato

ASJC Scopus subject areas

  • Virology
  • Molecular Biology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

A divergent isolate of Tomato yellow leaf curl virus from Oman with an associated DNAβ satellite : An evolutionary link between Asian and the Middle Eastern virus-satellite complexes. / Khan, Akhtar Jamal; Idris, Ali M.; Al-Saady, Nadiya Abubaker; Al-Mahruki, Madleen Said; Al-Subhi, Ali Masoud; Brown, J. K.

In: Virus Genes, Vol. 36, No. 1, 02.2008, p. 169-176.

Research output: Contribution to journalArticle

Khan, Akhtar Jamal ; Idris, Ali M. ; Al-Saady, Nadiya Abubaker ; Al-Mahruki, Madleen Said ; Al-Subhi, Ali Masoud ; Brown, J. K. / A divergent isolate of Tomato yellow leaf curl virus from Oman with an associated DNAβ satellite : An evolutionary link between Asian and the Middle Eastern virus-satellite complexes. In: Virus Genes. 2008 ; Vol. 36, No. 1. pp. 169-176.
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