A competitive nature-derived multilayered scaffold based on chitosan and alginate, for full-thickness wound healing

Saeid Vakilian, Fatemeh Jamshidi-adegani, Afra Al Yahmadi, Mohammed Al-Broumi, Najeeb Ur Rehman, Muhammad U. Anwar, Khurshid Alam, Nasar Al-Wahaibi, Asem Shalaby, Sausan Alyaqoobi, Ahmed Al-Harrasi, Kamal Mustafa, Sulaiman Al-Hashmi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


The aim of this study was to evaluate a bioactive multilayer wound dressing, based on chitosan and alginate. To enhance healing potential, Dracaena Cinnabari and Aloe Vera were loaded as separate layers into the scaffold. The bare and bioactive multilayered scaffolds were fabricated by an iterative layering freeze-drying technique. Following of topographical, chemical, and physical assessment, the performance of the scaffolds was evaluated in vitro and in vivo. The results revealed adequate attachment, and proliferation of human foreskin fibroblasts, indicating excellent biocompatibility of the bioactive scaffold. In vivo, the performance of the multi-layered scaffold loaded with the bioactive materials was comparable with Comfeel plus®. The wounds treated with the bioactive scaffold exhibited superior hypergranulation, fibroblast maturation, epithelization, and collagen deposition, with minimal inflammation, and crust formation. It is concluded that the synergism of extracellular matrix-mimicking multi-layered scaffolding with Aloe Vera and Dracaena Cinnabari could be considered as a supportive wound dressing.

Original languageEnglish
Article number117921
JournalCarbohydrate Polymers
Publication statusPublished - Jun 15 2021
Externally publishedYes


  • Alginate
  • Aloe vera
  • Chitosan
  • Dracaena Cinnabari
  • Multi-layered scaffold
  • Wound dressing

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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