A comparative study of interleukin-1β production and P2x7 expression after Atp stimulation by peripheral blood mononuclear cells isolated from rheumatoid arthritis patients and normal healthy controls

Ahmed Al-Shukaili, Juma Al-Kaabi, Batool Hassan

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Interleukin 1 beta (IL-1β) is a proinflammatory cytokine that is considered to play an important role in the progression of rheumatoid arthritis (RA). A stimulus such as ATP is necessary to cause the release of mature IL-1β, via activation of the P2X7 receptor on monocytes. In this study, the production of IL-1β in whole blood after ATP stimulation and expression of P2X7 receptors in RA and healthy subjects were examined. Blood samples from RA patients or healthy controls were stimulated with ATP in the presence of lipopolysaccharide (LPS). Supernatants were harvested and IL-1β levels were measured by enzyme-linked immunosorbent assay (ELISA). Expression of P2X7 receptors was measured using flow cytometry. ATP induced significantly higher levels of IL-1β in LPS-activated RA blood samples compared to controls. A significant up-regulation of P2X7 receptor expression on mononuclear cells was observed after overnight incubation with ATP without any significant differences between RA patients and normals. These data suggest that RA patient mononuclear cells are more sensitive to ATP stimulation than healthy individuals perhaps due to genetic polymorphism in the P2X7 gene.

Original languageEnglish
Pages (from-to)84-90
Number of pages7
JournalInflammation
Volume31
Issue number2
DOIs
Publication statusPublished - Apr 2008

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Interleukin-1
Purinergic P2X7 Receptors
Blood Cells
Rheumatoid Arthritis
Interleukin-1beta
Adenosine Triphosphate
Lipopolysaccharides
Genetic Polymorphisms
Monocytes
Healthy Volunteers
Flow Cytometry
Up-Regulation
Enzyme-Linked Immunosorbent Assay
Cytokines
Genes

Keywords

  • ATP
  • IL-1β
  • P2X receptors
  • RA

ASJC Scopus subject areas

  • Medicine(all)
  • Immunology
  • Cell Biology

Cite this

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abstract = "Interleukin 1 beta (IL-1β) is a proinflammatory cytokine that is considered to play an important role in the progression of rheumatoid arthritis (RA). A stimulus such as ATP is necessary to cause the release of mature IL-1β, via activation of the P2X7 receptor on monocytes. In this study, the production of IL-1β in whole blood after ATP stimulation and expression of P2X7 receptors in RA and healthy subjects were examined. Blood samples from RA patients or healthy controls were stimulated with ATP in the presence of lipopolysaccharide (LPS). Supernatants were harvested and IL-1β levels were measured by enzyme-linked immunosorbent assay (ELISA). Expression of P2X7 receptors was measured using flow cytometry. ATP induced significantly higher levels of IL-1β in LPS-activated RA blood samples compared to controls. A significant up-regulation of P2X7 receptor expression on mononuclear cells was observed after overnight incubation with ATP without any significant differences between RA patients and normals. These data suggest that RA patient mononuclear cells are more sensitive to ATP stimulation than healthy individuals perhaps due to genetic polymorphism in the P2X7 gene.",
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AB - Interleukin 1 beta (IL-1β) is a proinflammatory cytokine that is considered to play an important role in the progression of rheumatoid arthritis (RA). A stimulus such as ATP is necessary to cause the release of mature IL-1β, via activation of the P2X7 receptor on monocytes. In this study, the production of IL-1β in whole blood after ATP stimulation and expression of P2X7 receptors in RA and healthy subjects were examined. Blood samples from RA patients or healthy controls were stimulated with ATP in the presence of lipopolysaccharide (LPS). Supernatants were harvested and IL-1β levels were measured by enzyme-linked immunosorbent assay (ELISA). Expression of P2X7 receptors was measured using flow cytometry. ATP induced significantly higher levels of IL-1β in LPS-activated RA blood samples compared to controls. A significant up-regulation of P2X7 receptor expression on mononuclear cells was observed after overnight incubation with ATP without any significant differences between RA patients and normals. These data suggest that RA patient mononuclear cells are more sensitive to ATP stimulation than healthy individuals perhaps due to genetic polymorphism in the P2X7 gene.

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