A biosensor fabricated by incorporation of a redox mediator into a carbon nanotube/nafion composite for tyrosinase immobilization: Detection of matairesinol, an endocrine disruptor

Jahangir Ahmad Rather, Sanaz Pilehvar, Karolien De Wael

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21 Citations (Scopus)

Abstract

An electrochemical matairesinol biosensor was fabricated by immobilizing tyrosinase on a poly(thionine)/nafion/multi-walled carbon nanotube composite film. A polymeric film of the redox dye thionine enables the stable immobilization of tyrosinase while acting as a mediator for the enzymatic process has been incorporated into the carbon nanotube/nafion composite film. The immobilization method is based on crosslinking of the tyrosinase layer with an electropolymerized film of poly(thionine). The good homogenization of the electron conductor CNTs in the integrated films provides the possibility of a three-dimensional electron conductive network. The biosensor was characterized by electrochemical impedance spectroscopy and electrochemical characterization. The composite electrode exhibits catalytic activity, high sensitivity, stability and is applicable over a wide range of concentrations from 180 nM to 4.33 μM with a detection limit (LOD) of 37 nM. The obtained results suggest that the developed sensor can be successfully used for the determination of phenolic endocrine disruptors over a concentration range covering their environmental levels.

Original languageEnglish
Pages (from-to)204-210
Number of pages7
JournalAnalyst
Volume138
Issue number1
DOIs
Publication statusPublished - 2013

Fingerprint

Endocrine Disruptors
Carbon Nanotubes
endocrine disruptor
Monophenol Monooxygenase
Biosensing Techniques
Composite films
Biosensors
Immobilization
immobilization
Oxidation-Reduction
Carbon nanotubes
Electrons
Composite materials
Electrochemical impedance spectroscopy
Polymer films
Crosslinking
Dielectric Spectroscopy
Catalyst activity
Dyes
Electrodes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

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title = "A biosensor fabricated by incorporation of a redox mediator into a carbon nanotube/nafion composite for tyrosinase immobilization: Detection of matairesinol, an endocrine disruptor",
abstract = "An electrochemical matairesinol biosensor was fabricated by immobilizing tyrosinase on a poly(thionine)/nafion/multi-walled carbon nanotube composite film. A polymeric film of the redox dye thionine enables the stable immobilization of tyrosinase while acting as a mediator for the enzymatic process has been incorporated into the carbon nanotube/nafion composite film. The immobilization method is based on crosslinking of the tyrosinase layer with an electropolymerized film of poly(thionine). The good homogenization of the electron conductor CNTs in the integrated films provides the possibility of a three-dimensional electron conductive network. The biosensor was characterized by electrochemical impedance spectroscopy and electrochemical characterization. The composite electrode exhibits catalytic activity, high sensitivity, stability and is applicable over a wide range of concentrations from 180 nM to 4.33 μM with a detection limit (LOD) of 37 nM. The obtained results suggest that the developed sensor can be successfully used for the determination of phenolic endocrine disruptors over a concentration range covering their environmental levels.",
author = "Rather, {Jahangir Ahmad} and Sanaz Pilehvar and {De Wael}, Karolien",
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AU - Pilehvar, Sanaz

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