A 45-kDa chitinase purified from pearl millet (Pennisetum glaucum (L.) R. Br.) shows antifungal activity

R. Radhajeyalakshmi, B. Meena, R. Thangavelu, S. D. Deborah, P. Vidhyasekaran, R. Velazhahan

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Protein extracts from seeds of various crop plants were tested against Trichoderma viride. Among them, seed extracts of pearl millet (Pennisetum glaucum) was the most effective one in inhibiting the growth of T. viride in vitro. The highest chitinase activity was observed with pearl millet seed extract. Western blot analysis of protein extracts of pearl millet seeds using barley chitinase antiserum resulted in detection of a 30-kDa chitinase, whereas pearl millet florets constitutively expressed a 45-kDa chitinase. Puccinia penniseti infection and fungal elicitor treatment induced 45-kDa chitinase in pearl millet leaves. We have purified the 45-kDa chitinase from pearl millet florets using ammonium sulphate fractionation, chitin affinity chromatography followed by gel filtration on Sephadex G-75. The purified chitinase inhibited the growth of Rhizoctonia solani and T. viride.

Original languageEnglish
Pages (from-to)605-616
Number of pages12
JournalZeitschrift fur Pflanzenkrankheiten und Pflanzenschutz
Volume107
Issue number6
Publication statusPublished - 2000

Fingerprint

Pennisetum glaucum
chitinase
Trichoderma viride
seed extracts
florets
puccinia substriata var. penicillariae
extracts
Thanatephorus cucumeris
affinity chromatography
chitin
seeds
ammonium sulfate
antiserum
fractionation
Western blotting
proteins
barley
gels
crops
infection

Keywords

  • Antifungal chitinase
  • Elicitor
  • Pearl millet
  • Puccinia penniseti
  • Rhizoctonia solani
  • Trichoderma viride

ASJC Scopus subject areas

  • Plant Science

Cite this

A 45-kDa chitinase purified from pearl millet (Pennisetum glaucum (L.) R. Br.) shows antifungal activity. / Radhajeyalakshmi, R.; Meena, B.; Thangavelu, R.; Deborah, S. D.; Vidhyasekaran, P.; Velazhahan, R.

In: Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz, Vol. 107, No. 6, 2000, p. 605-616.

Research output: Contribution to journalArticle

Radhajeyalakshmi, R, Meena, B, Thangavelu, R, Deborah, SD, Vidhyasekaran, P & Velazhahan, R 2000, 'A 45-kDa chitinase purified from pearl millet (Pennisetum glaucum (L.) R. Br.) shows antifungal activity', Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz, vol. 107, no. 6, pp. 605-616.
Radhajeyalakshmi, R. ; Meena, B. ; Thangavelu, R. ; Deborah, S. D. ; Vidhyasekaran, P. ; Velazhahan, R. / A 45-kDa chitinase purified from pearl millet (Pennisetum glaucum (L.) R. Br.) shows antifungal activity. In: Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz. 2000 ; Vol. 107, No. 6. pp. 605-616.
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