454 pyrosequencing and direct plating reveal high fungal diversity and dominance by saprophytic species in organic compost

S. S. Al-Mazroui, A. M. Al-Sadi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

A study was conducted to investigate fungal diversity in organic compost originating from Oman. Analysis of diversity was conducted using 454 pyrosequencing and direct planting. The obtained fungal species through direct plating were identified based on the internal transcribed spacer region of the ribosomal RNA (rRNA). Pyrosequencing detected the presence of 94 fungal species, compared to 5 species detected by direct plating. Pyrosequencing also detected more fungal phyla, classes, orders, families and genera. Most of the detected species belonged to Ascomycota and Chytridiomycota, with Powellomyces spp., Eupenicillium spp. and Chaetomium spp. being the most dominant genera. The majority of the detected species (>99%) were found to be either saprophytic or with biocontrol characteristics, with few species (Fusarium and Phoma), being potential pathogens of plants. The low level of presence of pathogenic species may provide evidence of the health status of the organic compost. The study reports for the first time the occurrence of 67 fungal species in Oman. It discusses the superiority of pyrosequencing over direct plating and the factors influencing diversity of fungi in organic composts.

Original languageEnglish
Pages (from-to)98-102
Number of pages5
JournalInternational Journal of Agriculture and Biology
Volume18
Issue number1
DOIs
Publication statusPublished - 2016

Fingerprint

Oman
composts
Soil
Eupenicillium
Chytridiomycota
Chaetomium
Ascomycota
Phoma
Fusarium
health status
plant pathogens
internal transcribed spacers
Health Status
biological control
Fungi
planting
fungi

Keywords

  • Compost
  • Detection
  • Sequencing
  • Soil-borne pathogens

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

@article{fd3bb083672e4825a7b1229f9bc870da,
title = "454 pyrosequencing and direct plating reveal high fungal diversity and dominance by saprophytic species in organic compost",
abstract = "A study was conducted to investigate fungal diversity in organic compost originating from Oman. Analysis of diversity was conducted using 454 pyrosequencing and direct planting. The obtained fungal species through direct plating were identified based on the internal transcribed spacer region of the ribosomal RNA (rRNA). Pyrosequencing detected the presence of 94 fungal species, compared to 5 species detected by direct plating. Pyrosequencing also detected more fungal phyla, classes, orders, families and genera. Most of the detected species belonged to Ascomycota and Chytridiomycota, with Powellomyces spp., Eupenicillium spp. and Chaetomium spp. being the most dominant genera. The majority of the detected species (>99{\%}) were found to be either saprophytic or with biocontrol characteristics, with few species (Fusarium and Phoma), being potential pathogens of plants. The low level of presence of pathogenic species may provide evidence of the health status of the organic compost. The study reports for the first time the occurrence of 67 fungal species in Oman. It discusses the superiority of pyrosequencing over direct plating and the factors influencing diversity of fungi in organic composts.",
keywords = "Compost, Detection, Sequencing, Soil-borne pathogens",
author = "Al-Mazroui, {S. S.} and Al-Sadi, {A. M.}",
year = "2016",
doi = "10.17957/IJAB/15.0068",
language = "English",
volume = "18",
pages = "98--102",
journal = "International Journal of Agriculture and Biology",
issn = "1560-8530",
publisher = "Friends Science Publishers",
number = "1",

}

TY - JOUR

T1 - 454 pyrosequencing and direct plating reveal high fungal diversity and dominance by saprophytic species in organic compost

AU - Al-Mazroui, S. S.

AU - Al-Sadi, A. M.

PY - 2016

Y1 - 2016

N2 - A study was conducted to investigate fungal diversity in organic compost originating from Oman. Analysis of diversity was conducted using 454 pyrosequencing and direct planting. The obtained fungal species through direct plating were identified based on the internal transcribed spacer region of the ribosomal RNA (rRNA). Pyrosequencing detected the presence of 94 fungal species, compared to 5 species detected by direct plating. Pyrosequencing also detected more fungal phyla, classes, orders, families and genera. Most of the detected species belonged to Ascomycota and Chytridiomycota, with Powellomyces spp., Eupenicillium spp. and Chaetomium spp. being the most dominant genera. The majority of the detected species (>99%) were found to be either saprophytic or with biocontrol characteristics, with few species (Fusarium and Phoma), being potential pathogens of plants. The low level of presence of pathogenic species may provide evidence of the health status of the organic compost. The study reports for the first time the occurrence of 67 fungal species in Oman. It discusses the superiority of pyrosequencing over direct plating and the factors influencing diversity of fungi in organic composts.

AB - A study was conducted to investigate fungal diversity in organic compost originating from Oman. Analysis of diversity was conducted using 454 pyrosequencing and direct planting. The obtained fungal species through direct plating were identified based on the internal transcribed spacer region of the ribosomal RNA (rRNA). Pyrosequencing detected the presence of 94 fungal species, compared to 5 species detected by direct plating. Pyrosequencing also detected more fungal phyla, classes, orders, families and genera. Most of the detected species belonged to Ascomycota and Chytridiomycota, with Powellomyces spp., Eupenicillium spp. and Chaetomium spp. being the most dominant genera. The majority of the detected species (>99%) were found to be either saprophytic or with biocontrol characteristics, with few species (Fusarium and Phoma), being potential pathogens of plants. The low level of presence of pathogenic species may provide evidence of the health status of the organic compost. The study reports for the first time the occurrence of 67 fungal species in Oman. It discusses the superiority of pyrosequencing over direct plating and the factors influencing diversity of fungi in organic composts.

KW - Compost

KW - Detection

KW - Sequencing

KW - Soil-borne pathogens

UR - http://www.scopus.com/inward/record.url?scp=84958213222&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84958213222&partnerID=8YFLogxK

U2 - 10.17957/IJAB/15.0068

DO - 10.17957/IJAB/15.0068

M3 - Article

VL - 18

SP - 98

EP - 102

JO - International Journal of Agriculture and Biology

JF - International Journal of Agriculture and Biology

SN - 1560-8530

IS - 1

ER -