Abstract
A new fluorescent reagent for carboxylic acids, 4-N,N-dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-2,1,3-benz oxadiazole (DBD-ED) was synthesized from 4-N,N-dimethylaminosulfonyl-7-chloro-2,1,3-benzoxadiazole (DBD-Cl) and 1,2-diaminoethane. DBD-Cl is also a new compound described in this work for the first time. The applicability of DBD-ED as a precolumn derivatization reagent in liquid chromatography (LC) was examined. DBD-ED reacted with saturated fatty acids and non-steroidal anti-inflammatory drugs (NSAIDs) in the presence of triphenylphosphine and 2,2'-dipyridyl disulfide at room temperature for 30 min for NSAIDs and 1 h for fatty acids to give fluorescent products. The reaction solutions were subjected to LC and the derivatized products were detected spectrofluorimetrically at 560 nm with excitation at 450 nm. The detection limits were in the fmol range.
| Original language | English |
|---|---|
| Pages (from-to) | 227-232 |
| Number of pages | 6 |
| Journal | Analytica Chimica Acta |
| Volume | 344 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jun 10 1997 |
| Externally published | Yes |
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Keywords
- 2-Arylpropionic acid
- 4-N,N-Dimethylaminosulfonyl-7-chloro-2,1,3-benzoxadia zole (DBD-Cl)
- 4-N,N-Dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-2,1,3-benz oxadiazole (DBD-ED)
- Fatty acid
- Liquid chromatography
ASJC Scopus subject areas
- Biochemistry
- Analytical Chemistry
- Spectroscopy
- Environmental Chemistry
Cite this
4-N,N-Dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-benzofurazan as a new precolumn fluorescence derivatization reagent for carboxylic acids (fatty acids and drugs containing a carboxyl moiety) in liquid chromatography. / Prados, P.; Fukushima, T.; Santa, T.; Homma, H.; Tsunoda, M.; Al-Kindy, S.; Mori, S.; Yokosu, H.; Imai, K.
In: Analytica Chimica Acta, Vol. 344, No. 3, 10.06.1997, p. 227-232.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - 4-N,N-Dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-benzofurazan as a new precolumn fluorescence derivatization reagent for carboxylic acids (fatty acids and drugs containing a carboxyl moiety) in liquid chromatography
AU - Prados, P.
AU - Fukushima, T.
AU - Santa, T.
AU - Homma, H.
AU - Tsunoda, M.
AU - Al-Kindy, S.
AU - Mori, S.
AU - Yokosu, H.
AU - Imai, K.
PY - 1997/6/10
Y1 - 1997/6/10
N2 - A new fluorescent reagent for carboxylic acids, 4-N,N-dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-2,1,3-benz oxadiazole (DBD-ED) was synthesized from 4-N,N-dimethylaminosulfonyl-7-chloro-2,1,3-benzoxadiazole (DBD-Cl) and 1,2-diaminoethane. DBD-Cl is also a new compound described in this work for the first time. The applicability of DBD-ED as a precolumn derivatization reagent in liquid chromatography (LC) was examined. DBD-ED reacted with saturated fatty acids and non-steroidal anti-inflammatory drugs (NSAIDs) in the presence of triphenylphosphine and 2,2'-dipyridyl disulfide at room temperature for 30 min for NSAIDs and 1 h for fatty acids to give fluorescent products. The reaction solutions were subjected to LC and the derivatized products were detected spectrofluorimetrically at 560 nm with excitation at 450 nm. The detection limits were in the fmol range.
AB - A new fluorescent reagent for carboxylic acids, 4-N,N-dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-2,1,3-benz oxadiazole (DBD-ED) was synthesized from 4-N,N-dimethylaminosulfonyl-7-chloro-2,1,3-benzoxadiazole (DBD-Cl) and 1,2-diaminoethane. DBD-Cl is also a new compound described in this work for the first time. The applicability of DBD-ED as a precolumn derivatization reagent in liquid chromatography (LC) was examined. DBD-ED reacted with saturated fatty acids and non-steroidal anti-inflammatory drugs (NSAIDs) in the presence of triphenylphosphine and 2,2'-dipyridyl disulfide at room temperature for 30 min for NSAIDs and 1 h for fatty acids to give fluorescent products. The reaction solutions were subjected to LC and the derivatized products were detected spectrofluorimetrically at 560 nm with excitation at 450 nm. The detection limits were in the fmol range.
KW - 2-Arylpropionic acid
KW - 4-N,N-Dimethylaminosulfonyl-7-chloro-2,1,3-benzoxadia zole (DBD-Cl)
KW - 4-N,N-Dimethylaminosulfonyl-7-N-(2-aminoethyl)amino-2,1,3-benz oxadiazole (DBD-ED)
KW - Fatty acid
KW - Liquid chromatography
UR - http://www.scopus.com/inward/record.url?scp=0031006479&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031006479&partnerID=8YFLogxK
U2 - 10.1016/S0003-2670(97)00020-2
DO - 10.1016/S0003-2670(97)00020-2
M3 - Article
VL - 344
SP - 227
EP - 232
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
SN - 0003-2670
IS - 3
ER -