TY - JOUR
T1 - Usutu virus growth in human cell lines
T2 - Induction of and sensitivity to type I and III interferons
AU - Scagnolari, Carolina
AU - Caputo, Beniamino
AU - Trombetti, Simona
AU - Cacciotti, Giulia
AU - Soldà, Annalisa
AU - Spano, Lucia
AU - Villari, Paolo
AU - della Torre, Alessandra
AU - Nowotny, Norbert
AU - Antonelli, Guido
PY - 2013/4
Y1 - 2013/4
N2 - The mechanisms of Usutu virus (USUV) pathogenesis are largely unknown. The aim of this study was to evaluate the sensitivity of USUV to interferon (IFN) and the capacity of USUV to stimulate IFN production. Initial experiments were conducted to characterize the susceptibility of human cell lines to USUV infection and to evaluate the single-growth cycle replication curve of USUV. Results indicate that USUV is able to infect a variety of human cell lines, completing the replication cycle in Hep-2 and Vero cells within 48 h. Pre-treatment of cells with types I and III IFNs significantly inhibited the replication of USUV. However, the inhibitory effects of IFNs were considerably less if IFN was added after viral infection had been initiated. Also, USUV weakly induced types I and III IFNs.
AB - The mechanisms of Usutu virus (USUV) pathogenesis are largely unknown. The aim of this study was to evaluate the sensitivity of USUV to interferon (IFN) and the capacity of USUV to stimulate IFN production. Initial experiments were conducted to characterize the susceptibility of human cell lines to USUV infection and to evaluate the single-growth cycle replication curve of USUV. Results indicate that USUV is able to infect a variety of human cell lines, completing the replication cycle in Hep-2 and Vero cells within 48 h. Pre-treatment of cells with types I and III IFNs significantly inhibited the replication of USUV. However, the inhibitory effects of IFNs were considerably less if IFN was added after viral infection had been initiated. Also, USUV weakly induced types I and III IFNs.
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U2 - 10.1099/vir.0.046433-0
DO - 10.1099/vir.0.046433-0
M3 - Article
C2 - 23255619
AN - SCOPUS:84875682341
SN - 0022-1317
VL - 94
SP - 789
EP - 795
JO - Journal of General Virology
JF - Journal of General Virology
IS - PART4
ER -