TY - JOUR
T1 - Two Cultivars of Ocimum basilicum Leaves Extracts Attenuate Streptozotocin-mediated Oxidative Stress in Diabetic Rats
AU - Al-Subhi, Lyutha
AU - Waly, Mostafa Ibrahim
N1 - Publisher Copyright:
© 2020 Lyutha Al-Subhi and Mostafa Ibrahim Waly.
PY - 2020/1
Y1 - 2020/1
N2 - BACKGROUND AND OBJECTIVE: Leaves of basil plant (Ocimum basilicum) are often used for medicinal purposes because of their bioactive constituents, yet the antioxidant properties of this plant are not fully studied in the field of diabetes. The present study investigated the antioxidant property and anti-diabetic effect of two basil cultivars of Ocimum basilicum, 'Italian Genovese' and 'Thyrsiflora', in a Streptozotocin (STZ) rat model of diabetes .MATERIALS AND METHODS: Sixty adult Sprague Dawley rats (n = 10/group) were divided into 6 groups: Three non-diabetic and three diabetic groups that either did not receive any supplementation or were supplemented with the leaves extract of one or the other cultivar. After 13 weeks of feeding, all rats were sacrificed, pancreatic tissues were homogenized and used for evaluating oxidative DNA damage and dichlorofluorescein fluorescence (DCF) assay. Blood was collected for the measurements of glucose and insulin.RESULTS: The STZ caused oxidative stress in the diabetic group as evidenced by an increase in oxidative DNA damage and also caused DCF production in pancreatic tissues as compared to non-diabetic groups, (p<0.05). The STZ treatment resulted in hyperglycemia and low serum insulin level in diabetic rats. Supplementation with extracts of 'Italian Genovese' and 'Thyrsiflora' to the diabetic groups significantly abrogated the STZ-mediated effects (p<0.05).CONCLUSION: The results indicated that the extracts from the leaves of the two examined basil cultivars act as potent antioxidants and combat the STZ-mediated diabetogenic effect.
AB - BACKGROUND AND OBJECTIVE: Leaves of basil plant (Ocimum basilicum) are often used for medicinal purposes because of their bioactive constituents, yet the antioxidant properties of this plant are not fully studied in the field of diabetes. The present study investigated the antioxidant property and anti-diabetic effect of two basil cultivars of Ocimum basilicum, 'Italian Genovese' and 'Thyrsiflora', in a Streptozotocin (STZ) rat model of diabetes .MATERIALS AND METHODS: Sixty adult Sprague Dawley rats (n = 10/group) were divided into 6 groups: Three non-diabetic and three diabetic groups that either did not receive any supplementation or were supplemented with the leaves extract of one or the other cultivar. After 13 weeks of feeding, all rats were sacrificed, pancreatic tissues were homogenized and used for evaluating oxidative DNA damage and dichlorofluorescein fluorescence (DCF) assay. Blood was collected for the measurements of glucose and insulin.RESULTS: The STZ caused oxidative stress in the diabetic group as evidenced by an increase in oxidative DNA damage and also caused DCF production in pancreatic tissues as compared to non-diabetic groups, (p<0.05). The STZ treatment resulted in hyperglycemia and low serum insulin level in diabetic rats. Supplementation with extracts of 'Italian Genovese' and 'Thyrsiflora' to the diabetic groups significantly abrogated the STZ-mediated effects (p<0.05).CONCLUSION: The results indicated that the extracts from the leaves of the two examined basil cultivars act as potent antioxidants and combat the STZ-mediated diabetogenic effect.
KW - Animals
KW - Blood Glucose/analysis
KW - Diabetes Mellitus, Experimental/drug therapy
KW - Hypoglycemic Agents/pharmacology
KW - Insulin/blood
KW - Male
KW - Ocimum basilicum/chemistry
KW - Oxidative Stress/drug effects
KW - Plant Extracts/pharmacology
KW - Plant Leaves/chemistry
KW - Rats
KW - Rats, Sprague-Dawley
KW - Streptozocin
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U2 - 10.3923/pjbs.2020.1010.1017
DO - 10.3923/pjbs.2020.1010.1017
M3 - Article
C2 - 32700851
AN - SCOPUS:85088243104
SN - 1028-8880
VL - 23
SP - 1010
EP - 1017
JO - Pakistan Journal of Biological Sciences
JF - Pakistan Journal of Biological Sciences
IS - 8
ER -