The expression of p53, p21, Bax and induction of apoptosis in normal volunteers in response to different doses of ultraviolet radiation

M. Murphy, M. J.E.M.F. Mabruk, P. Lenane, A. Liew, P. Mccann, A. Buckley, P. Billet, M. Leader, E. Kay, G. M. Murphy*

*المؤلف المقابل لهذا العمل

نتاج البحث: المساهمة في مجلةArticleمراجعة النظراء

38 اقتباسات (Scopus)

ملخص

Background: Ultraviolet radiation (UVR) damages keratinocytes. Direct DNA damage may undergo enzymatic repair followed by resumption of the normal cell cycle. Cells may also be eliminated without inflammation by the error-free process of programmed cell death or apoptosis. Necrosis of cells can occur after overwhelming damage. Failure of apoptosis leads to retention of cells with persistent mutations. Objectives: This study investigates p53-dependent apoptotic responses in normal skin following solar-simulated radiation (SSR). Methods: Sun-protected buttock skin from normal volunteers with no history or clinical evidence of skin cancer was exposed to graded doses of SSR, 0.5, 1, 2 and 3 times the minimal erythema dose (MED). Biopsies taken at a range of time points (4.5, 9, 24, 33, 48 and 72 h) after UVR, quantified the time course and dose-response of apoptosis and the expression of the relevant proteins, p53, p21waf1/Cip1 and Bax, by single and double labelling techniques. Results: Apoptosis was upregulated in a dose-dependent manner as was the expression of p53, p21waf1/Cip1 and Bax in response to SSR. Following exposure to 3 MEDs it was found that: (i) the maximum number of apoptotic cells occurred at 48 h; (ii) p53 protein expression was upregulated from 4 to 72 h preceding peak p21waf1/Cip1 protein expression (9-48 h) and peak Bax protein expression (33 h). Conclusions: These results suggest that, following SSR, normal human skin induces apoptosis by the p53, p21waf1/Cip1, Bax pathway in vivo. In addition, induction of apoptosis and expression of p53, p21waf1/Cip1 and Bax occurs in a dose-dependent manner.

اللغة الأصليةEnglish
الصفحات (من إلى)110-117
عدد الصفحات8
دوريةBritish Journal of Dermatology
مستوى الصوت147
رقم الإصدار1
المعرِّفات الرقمية للأشياء
حالة النشرPublished - 2002
منشور خارجيًانعم

ASJC Scopus subject areas

  • ???subjectarea.asjc.2700.2708???

بصمة

أدرس بدقة موضوعات البحث “The expression of p53, p21, Bax and induction of apoptosis in normal volunteers in response to different doses of ultraviolet radiation'. فهما يشكلان معًا بصمة فريدة.

قم بذكر هذا