TY - JOUR
T1 - The colocalization potential of HIV-specific CD8 + and CD4 + T-cells is mediated by integrin β7 but not CCR6 and regulated by retinoic acid
AU - Wacleche, Vanessa Sue
AU - Chomont, Nicolas
AU - Gosselin, Annie
AU - Monteiro, Patricia
AU - Goupil, Mathieu
AU - Kared, Hassen
AU - Tremblay, Cécile
AU - Bernard, Nicole
AU - Boulassel, Mohamed Rachid
AU - Routy, Jean Pierre
AU - Ancuta, Petronela
PY - 2012/3/28
Y1 - 2012/3/28
N2 - CD4 + T-cells from gut-associated lymphoid tissues (GALT) are major targets for HIV-1 infection. Recruitment of excess effector CD8 + T-cells in the proximity of target cells is critical for the control of viral replication. Here, we investigated the colocalization potential of HIV-specific CD8 + and CD4 + T-cells into the GALT and explored the role of retinoic acid (RA) in regulating this process in a cohort of HIV-infected subjects with slow disease progression. The expression of the gut-homing molecules integrin β7, CCR6, and CXCR3 was identified as a "signature" for HIV-specific but not CMV-specific CD4 + T-cells thus providing a new explanation for their enhanced permissiveness to infection in vivo. HIV-specific CD8 + T-cells also expressed high levels of integrin β7 and CXCR3; however CCR6 was detected at superior levels on HIV-specific CD4 + versus CD8 + T-cells. All trans RA (ATRA) upregulated the expression of integrin β7 but not CCR6 on HIV-specific T-cells. Together, these results suggest that HIV-specific CD8 + T-cells may colocalize in excess with CD4 + T-cells into the GALT via integrin β7 and CXCR3, but not via CCR6. Considering our previous findings that CCR6 +CD4 + T-cells are major cellular targets for HIV-DNA integration in vivo, a limited ability of CD8 + T-cells to migrate in the vicinity of CCR6 +CD4 + T-cells may facilitate HIV replication and dissemination at mucosal sites.
AB - CD4 + T-cells from gut-associated lymphoid tissues (GALT) are major targets for HIV-1 infection. Recruitment of excess effector CD8 + T-cells in the proximity of target cells is critical for the control of viral replication. Here, we investigated the colocalization potential of HIV-specific CD8 + and CD4 + T-cells into the GALT and explored the role of retinoic acid (RA) in regulating this process in a cohort of HIV-infected subjects with slow disease progression. The expression of the gut-homing molecules integrin β7, CCR6, and CXCR3 was identified as a "signature" for HIV-specific but not CMV-specific CD4 + T-cells thus providing a new explanation for their enhanced permissiveness to infection in vivo. HIV-specific CD8 + T-cells also expressed high levels of integrin β7 and CXCR3; however CCR6 was detected at superior levels on HIV-specific CD4 + versus CD8 + T-cells. All trans RA (ATRA) upregulated the expression of integrin β7 but not CCR6 on HIV-specific T-cells. Together, these results suggest that HIV-specific CD8 + T-cells may colocalize in excess with CD4 + T-cells into the GALT via integrin β7 and CXCR3, but not via CCR6. Considering our previous findings that CCR6 +CD4 + T-cells are major cellular targets for HIV-DNA integration in vivo, a limited ability of CD8 + T-cells to migrate in the vicinity of CCR6 +CD4 + T-cells may facilitate HIV replication and dissemination at mucosal sites.
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U2 - 10.1371/journal.pone.0032964
DO - 10.1371/journal.pone.0032964
M3 - Article
C2 - 22470433
AN - SCOPUS:84859068534
SN - 1932-6203
VL - 7
JO - PLoS One
JF - PLoS One
IS - 3
M1 - e32964
ER -