Streptomyces lividans and Brevibacterium lactofermentum as heterologous hosts for the production of X22 xylanase from Aspergillus nidulans

M. Díaz, S. A.I. Adham, D. Ramón, J. A. Gil, R. I. Santamaría*

*المؤلف المقابل لهذا العمل

نتاج البحث: المساهمة في مجلةمراجعة النظراء

16 اقتباسات (Scopus)

ملخص

The Aspergillus nidulans gene xlnA coding for the fungal xylanase X 22 has been cloned and expressed in two heterologous bacterial hosts: Streptomyces lividans and Brevibacterium lactofermentum. Streptomyces strains yielded 10 units/ml of xylanase when the protein was produced with its own signal peptide, and 19 units/ml when its signal peptide was replaced by the one for xylanase Xys1 from Streptomyces halstedii. B. lactofermentum was also able to produce xylanase X22, affording 6 units/ml upon using either the Aspergillus xlnA signal peptide or Streptomyces xysA. These production values are higher than those previously reported for the heterologous expression of the A. nidulans xlnA gene in Saccharomyces cerevisiae (1 unit/ml). Moreover, the X22 enzyme produced by Streptomyces lividans showed oenological properties, indicating that this Streptomyces recombinant strain is a good candidate for the production of this enzyme at the industrial scale.

اللغة الأصليةEnglish
الصفحات (من إلى)401-406
عدد الصفحات6
دوريةApplied Microbiology and Biotechnology
مستوى الصوت65
رقم الإصدار4
المعرِّفات الرقمية للأشياء
حالة النشرPublished - سبتمبر 2004
منشور خارجيًانعم

ASJC Scopus subject areas

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بصمة

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