TY - JOUR
T1 - Selective inhibition of pressor and haemodynamic effects of ng-nitro-l-arginine by halothane
AU - Wang, Yong Xiang
AU - Abdelrahman, Aly
AU - Pang, Catherine C.Y.
PY - 1993/10
Y1 - 1993/10
N2 - We investigated the characteristics of inhibition by halothane of the pressor responses to NG-substituted L-arginine derivatives, nitric oxide (NO) synthase inhibitors. Intravenous (i.v.) bolus injections of NG-nitro-L-arginine (L-NNA, 1–32 mg/kg), NG-nitro-L-arginine methyl ester (L-NAME, 0.4–12.8 mg/kg), norepinephrine (NE, 0.25–8 (μg/kg) and angiotensin II (AH, 0.02–0.64 μg/kg) each caused dose-dependent pressor responses in conscious rats. Halothane attenuated responses to the highest dose of NE and AH by 18% but completely abolished responses to L-NNA and L-NAME. The haemodynamic effects of L-NNA were further examined by the microsphere technique in two groups of conscious rats and two groups of halothane-anaesthetized rats. An i.v. bolus injection of L-NNA (16 mg/kg) in conscious rats increased mean arterial pressure (MAP) and total peripheral resistance (TPR) and reduced heart rate (HR) and cardiac output (CO). These changes were associated with reduced conductance in all vascular beds, with the greatest reduction in the lungs and the least in the liver. In halothane-anaesthetized rats, L-NNA caused significant but markedly less change in MAP, HR, TPR, and CO as compared with those in conscious rats. The vasoconstrictor effects of L-NNA were attenuated by halothane in all beds except liver and spleen, with the greatest inhibition in heart. Our results suggest that NO plays a role in maintenance of peripheral vascular resistance and that halothane selectively and “noncompetitively” inhibits the vasoconstrictor effects of NO synthase inhibitors.
AB - We investigated the characteristics of inhibition by halothane of the pressor responses to NG-substituted L-arginine derivatives, nitric oxide (NO) synthase inhibitors. Intravenous (i.v.) bolus injections of NG-nitro-L-arginine (L-NNA, 1–32 mg/kg), NG-nitro-L-arginine methyl ester (L-NAME, 0.4–12.8 mg/kg), norepinephrine (NE, 0.25–8 (μg/kg) and angiotensin II (AH, 0.02–0.64 μg/kg) each caused dose-dependent pressor responses in conscious rats. Halothane attenuated responses to the highest dose of NE and AH by 18% but completely abolished responses to L-NNA and L-NAME. The haemodynamic effects of L-NNA were further examined by the microsphere technique in two groups of conscious rats and two groups of halothane-anaesthetized rats. An i.v. bolus injection of L-NNA (16 mg/kg) in conscious rats increased mean arterial pressure (MAP) and total peripheral resistance (TPR) and reduced heart rate (HR) and cardiac output (CO). These changes were associated with reduced conductance in all vascular beds, with the greatest reduction in the lungs and the least in the liver. In halothane-anaesthetized rats, L-NNA caused significant but markedly less change in MAP, HR, TPR, and CO as compared with those in conscious rats. The vasoconstrictor effects of L-NNA were attenuated by halothane in all beds except liver and spleen, with the greatest inhibition in heart. Our results suggest that NO plays a role in maintenance of peripheral vascular resistance and that halothane selectively and “noncompetitively” inhibits the vasoconstrictor effects of NO synthase inhibitors.
KW - Blood pressure
KW - Haemo-dynamics
KW - Halothane
KW - Ng-nitro-l-arginine
KW - Ng-nitro-l-arginine methyl ester
KW - Nitric oxide
KW - Rat
KW - Vascular conductance
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U2 - 10.1097/00005344-199310000-00010
DO - 10.1097/00005344-199310000-00010
M3 - Article
C2 - 7505359
AN - SCOPUS:0027491269
SN - 0160-2446
VL - 22
SP - 577
EP - 578
JO - Journal of Cardiovascular Pharmacology
JF - Journal of Cardiovascular Pharmacology
IS - 4
ER -