TY - JOUR
T1 - Role of adhatoda vasica (L.) nees leaf extract in the prevention of aflatoxin-induced toxicity in Wistar rats
AU - Brinda, Rajendran
AU - Vijayanandraj, Selvaraj
AU - Uma, Doraiswamy
AU - Malathi, Dorairaj
AU - Paranidharan, Vaikuntavasan
AU - Velazhahan, Rethinasamy
PY - 2013/8/30
Y1 - 2013/8/30
N2 - Background: Aflatoxin contamination of various foodstuffs and agricultural commodities is a major problem worldwide. Several strategies have been reported for the detoxification of aflatoxins in contaminated foods and feeds, but all these methods have their own shortcomings. Traditional medicinal plants are potential sources of aflatoxin-detoxifying compounds. In this study a spray-dried formulation of Adhatoda vasica (L.) Nees leaf extract was prepared and its chemopreventive effect on aflatoxin B1 (AFB1)-induced biochemical changes in the liver and serum of Wistar rats was investigated. Results: Administration of AFB1 (1.5 mg kg-1 body weight (BW) intraperitoneally) to rats significantly reduced the activities of superoxide dismutase and catalase in liver tissues and increased the activities of aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase and the levels of very-low-density lipoprotein, low-density lipoprotein and cholesterol in blood serum. However, pre-feeding of rats with A. vasica formulation (500 mg kg-1 BW for 7 days) protected the animals from AFB1-induced biochemical changes during subsequent exposure to AFB1. Conclusion: Pre-feeding of rats with A. vasica formulation counteracted the hepatic dysfunction induced by subsequent treatment with AFB1. This formulated A. vasica extract offers a biologically safe alternative to detoxify aflatoxin and has huge potential to be used in the poultry industry to reduce aflatoxicosis.
AB - Background: Aflatoxin contamination of various foodstuffs and agricultural commodities is a major problem worldwide. Several strategies have been reported for the detoxification of aflatoxins in contaminated foods and feeds, but all these methods have their own shortcomings. Traditional medicinal plants are potential sources of aflatoxin-detoxifying compounds. In this study a spray-dried formulation of Adhatoda vasica (L.) Nees leaf extract was prepared and its chemopreventive effect on aflatoxin B1 (AFB1)-induced biochemical changes in the liver and serum of Wistar rats was investigated. Results: Administration of AFB1 (1.5 mg kg-1 body weight (BW) intraperitoneally) to rats significantly reduced the activities of superoxide dismutase and catalase in liver tissues and increased the activities of aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase and the levels of very-low-density lipoprotein, low-density lipoprotein and cholesterol in blood serum. However, pre-feeding of rats with A. vasica formulation (500 mg kg-1 BW for 7 days) protected the animals from AFB1-induced biochemical changes during subsequent exposure to AFB1. Conclusion: Pre-feeding of rats with A. vasica formulation counteracted the hepatic dysfunction induced by subsequent treatment with AFB1. This formulated A. vasica extract offers a biologically safe alternative to detoxify aflatoxin and has huge potential to be used in the poultry industry to reduce aflatoxicosis.
KW - Adhatoda vasica
KW - Aflatoxin
KW - Chemoprevention
KW - Detoxification
KW - Medicinal plants
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U2 - 10.1002/jsfa.6093
DO - 10.1002/jsfa.6093
M3 - Article
C2 - 23519623
AN - SCOPUS:84880166863
SN - 0022-5142
VL - 93
SP - 2743
EP - 2748
JO - Journal of the Science of Food and Agriculture
JF - Journal of the Science of Food and Agriculture
IS - 11
ER -