TY - JOUR
T1 - Recent Trends and Advancements in CRISPR-Based Tools for Enhancing Resistance against Plant Pathogens
AU - Ijaz, Munazza
AU - Khan, Fahad
AU - Zaki, Haitham E.M.
AU - Khan, Muhammad Munem
AU - Radwan, Khlode S.A.
AU - Jiang, Yugen
AU - Qian, Jiahui
AU - Ahmed, Temoor
AU - Shahid, Muhammad Shafiq
AU - Luo, Jinyan
AU - Li, Bin
N1 - Publisher Copyright:
© 2023 by the authors.
PY - 2023/5/8
Y1 - 2023/5/8
N2 - Targeted genome editing technologies are becoming the most important and widely used genetic tools in studies of phytopathology. The “clustered regularly interspaced short palindromic repeats (CRISPR)” and its accompanying proteins (Cas) have been first identified as a natural system associated with the adaptive immunity of prokaryotes that have been successfully used in various genome-editing techniques because of its flexibility, simplicity, and high efficiency in recent years. In this review, we have provided a general idea about different CRISPR/Cas systems and their uses in phytopathology. This review focuses on the benefits of knock-down technologies for targeting important genes involved in the susceptibility and gaining resistance against viral, bacterial, and fungal pathogens by targeting the negative regulators of defense pathways of hosts in crop plants via different CRISPR/Cas systems. Moreover, the possible strategies to employ CRISPR/Cas system for improving pathogen resistance in plants and studying plant–pathogen interactions have been discussed.
AB - Targeted genome editing technologies are becoming the most important and widely used genetic tools in studies of phytopathology. The “clustered regularly interspaced short palindromic repeats (CRISPR)” and its accompanying proteins (Cas) have been first identified as a natural system associated with the adaptive immunity of prokaryotes that have been successfully used in various genome-editing techniques because of its flexibility, simplicity, and high efficiency in recent years. In this review, we have provided a general idea about different CRISPR/Cas systems and their uses in phytopathology. This review focuses on the benefits of knock-down technologies for targeting important genes involved in the susceptibility and gaining resistance against viral, bacterial, and fungal pathogens by targeting the negative regulators of defense pathways of hosts in crop plants via different CRISPR/Cas systems. Moreover, the possible strategies to employ CRISPR/Cas system for improving pathogen resistance in plants and studying plant–pathogen interactions have been discussed.
KW - CRISPR
KW - genome editing
KW - phytopathology
KW - resistance
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UR - https://www.mendeley.com/catalogue/350c6cc9-eb1e-3e68-8c0e-4de938b71b91/
U2 - 10.3390/plants12091911
DO - 10.3390/plants12091911
M3 - Review article
C2 - 37176969
AN - SCOPUS:85159174451
SN - 2223-7747
VL - 12
JO - Plants
JF - Plants
IS - 9
M1 - 1911
ER -