TY - JOUR
T1 - Overexpression of thaumatin-like protein in transgenic tomato plants confers enhanced resistance to Alternaria solani
AU - Radhajeyalakshmi, Raju
AU - Velazhahan, Rethinasamy
AU - Balasubramanian, Ponnusamy
AU - Doraiswamy, Sabitha
N1 - Funding Information:
This work was supported by the Council for Scientific and Industrial Research, New Delhi, India.
PY - 2005/11
Y1 - 2005/11
N2 - A cDNA encoding thaumatin-like protein (TLP) from rice was cloned into the binary vector pMON410 under the control of the CaMV 35S promoter for Agrobacterium-mediated transformation of tomato. All putative transformants were tested for the integration and expression of the chimeric gene by polymerase chain reaction (PCR) for hygromycin resistance gene (hph) and enzyme-linked immunosorbent assay (ELISA) for TLP respectively. Constitutive, high-level expression of TLP was observed in transgenic plants. The transgenic lines exhibited increased resistance to Alternaria solani, the early blight pathogen compared to non-transgenic tomato plants.
AB - A cDNA encoding thaumatin-like protein (TLP) from rice was cloned into the binary vector pMON410 under the control of the CaMV 35S promoter for Agrobacterium-mediated transformation of tomato. All putative transformants were tested for the integration and expression of the chimeric gene by polymerase chain reaction (PCR) for hygromycin resistance gene (hph) and enzyme-linked immunosorbent assay (ELISA) for TLP respectively. Constitutive, high-level expression of TLP was observed in transgenic plants. The transgenic lines exhibited increased resistance to Alternaria solani, the early blight pathogen compared to non-transgenic tomato plants.
KW - Disease resistance
KW - Lycopersicon esculentum
KW - Pathogenesis-related proteins
KW - Thaumatin-like protein
KW - Transgenic plants
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U2 - 10.1080/03235400400027382
DO - 10.1080/03235400400027382
M3 - Article
AN - SCOPUS:29944446529
SN - 0323-5408
VL - 38
SP - 257
EP - 265
JO - Archives of Phytopathology and Plant Protection
JF - Archives of Phytopathology and Plant Protection
IS - 4
ER -