TY - CHAP
T1 - Multiplex Testing of the Effect of Statins on Disease Severity Risk in COVID-19 Cases
AU - Zahedipour, Fatemeh
AU - Guest, Paul C.
AU - Majeed, Muhammed
AU - Al-Rasadi, Khalid
AU - Jamialahmadi, Tannaz
AU - Sahebkar, Amirhossein
N1 - Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2022/1/1
Y1 - 2022/1/1
N2 - Statins have pleiotropic effects on inflammatory responses in addition to their lipid-lowering action, which contributes to their favorable effect on cardiovascular disorders. Statins affect adhesion, migration, antigen presentation, and cytokine generation of immune cells. Pre-clinical and clinical studies suggest that statin intervention targeted early in the infection might help COVID-19 patients to reduce the effects of acute respiratory distress syndrome (ARDS), the cytokine storm, and vascular collapse by modulating harmful pathogenic mechanisms. This chapter presents a protocol for measuring blood-based biomarkers predictive of these responses in COVID-19 patients using two specific multiplex immunoassays that target proteins that differ widely in concentration.
AB - Statins have pleiotropic effects on inflammatory responses in addition to their lipid-lowering action, which contributes to their favorable effect on cardiovascular disorders. Statins affect adhesion, migration, antigen presentation, and cytokine generation of immune cells. Pre-clinical and clinical studies suggest that statin intervention targeted early in the infection might help COVID-19 patients to reduce the effects of acute respiratory distress syndrome (ARDS), the cytokine storm, and vascular collapse by modulating harmful pathogenic mechanisms. This chapter presents a protocol for measuring blood-based biomarkers predictive of these responses in COVID-19 patients using two specific multiplex immunoassays that target proteins that differ widely in concentration.
KW - C-reactive protein
KW - COVID-19
KW - IL-6
KW - IL-8
KW - Multiplex immunoassay
KW - SARS-CoV-2
KW - Simvastatin
KW - Statins
KW - TNF-α
KW - Von Willebrand factor
KW - Cytokine Release Syndrome
KW - Severity of Illness Index
KW - COVID-19 Drug Treatment
KW - Humans
KW - Hydroxymethylglutaryl-CoA Reductase Inhibitors/pharmacology
UR - http://www.scopus.com/inward/record.url?scp=85134588332&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85134588332&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/d7a58891-de3b-3112-8e51-d451eea3592a/
U2 - 10.1007/978-1-0716-2395-4_20
DO - 10.1007/978-1-0716-2395-4_20
M3 - Chapter
C2 - 35838967
AN - SCOPUS:85134588332
VL - 2511
T3 - Methods in molecular biology (Clifton, N.J.)
SP - 273
EP - 284
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -