ملخص
Recrudescent Plasmodium falciparum parasites were sampled from 108 children taking part in a drug efficacy trial in Gabon. A finger-prick blood sample was taken from each child before treatment, and a post-treatment sample taken of the recrudescent parasites. Sample deoxyribonucleic acid was amplified by the polymerase chain reaction using primers specific to the P. falciparum antigen genes MSP-1, MSP-2 and GLURP. Seventy-seven children had identical parasites in their pre- and post-treatment samples, indicating genuine recrudescences of resistant parasites. Fourteen children had completely different parasites in their pre- and post-treatment samples, indicating either a fresh infection from a mosquito or growth of a population of parasites not detected in the pre-treatment sample, perhaps due to sequestration. The remaining 17 children had a mixture of pre-treatment and new parasites in their post-treatment samples. This study demonstrated the use of polymorphic markers to confirm whether parasites in patients with clinical recrudescences after drug treatment are genuinely resistant.
اللغة الأصلية | English |
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الصفحات (من إلى) | 719-724 |
عدد الصفحات | 6 |
دورية | Transactions of the Royal Society of Tropical Medicine and Hygiene |
مستوى الصوت | 91 |
رقم الإصدار | 6 |
المعرِّفات الرقمية للأشياء | |
حالة النشر | Published - 1997 |
منشور خارجيًا | نعم |
ASJC Scopus subject areas
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