TY - JOUR
T1 - Microfluidic precolumn derivatization of environmental phenols with coumarin-6-sulfonyl chloride and HPLC separation
AU - Suliman, Fakhreldin O.
AU - Al-Busaidi, Jihad N.
AU - Al-Lawati, Hiader A.J.
AU - Al-Kindy, Salma M.Z.
N1 - Publisher Copyright:
© The Author 2015. Published by Oxford University Press. All rights reserved.
PY - 2015/9
Y1 - 2015/9
N2 - A simple, fast, sensitive and versatile method for the analysis of phenols in water is proposed using microfluidic precolumn derivatization with the fluorogenic label coumarin-6-sulfonyl chloride (C6SCl) and HPLC separation on monolithic columns. Phenols react with C6SCl within 3.0 min in the microreactor at ambient temperature to produce phenol-coumarin sulphonamides derivatives which were separated in reversed phase high-performance liquid chromatography followed by postcolumn ring-opening and fluorescence detection at lexc 5 360 nm and lem 5 460 nm. The optimum conditions for the derivatization, separation and ring-opening reaction have been established. The calibration curves were linear for the studied phenols in the range of 0.75-12.5 mg L21. The application of the method to environmental samples was demonstrated by analyzing tap and fountain water samples spiked with the phenolic compounds.
AB - A simple, fast, sensitive and versatile method for the analysis of phenols in water is proposed using microfluidic precolumn derivatization with the fluorogenic label coumarin-6-sulfonyl chloride (C6SCl) and HPLC separation on monolithic columns. Phenols react with C6SCl within 3.0 min in the microreactor at ambient temperature to produce phenol-coumarin sulphonamides derivatives which were separated in reversed phase high-performance liquid chromatography followed by postcolumn ring-opening and fluorescence detection at lexc 5 360 nm and lem 5 460 nm. The optimum conditions for the derivatization, separation and ring-opening reaction have been established. The calibration curves were linear for the studied phenols in the range of 0.75-12.5 mg L21. The application of the method to environmental samples was demonstrated by analyzing tap and fountain water samples spiked with the phenolic compounds.
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U2 - 10.1093/chromsci/bmv028
DO - 10.1093/chromsci/bmv028
M3 - Article
C2 - 25809998
AN - SCOPUS:84953738102
SN - 0021-9665
VL - 53
SP - 1379
EP - 1385
JO - Journal of Chromatographic Science
JF - Journal of Chromatographic Science
IS - 8
ER -