TY - JOUR
T1 - Mechanistic Approaches of PHE and PPF Columns for Separation of Rasberry Ketone and Caffeine
AU - Alothman, Zeid A.
AU - Al-Warthan, Abdulrahman
AU - Aboul-Enein, Hassan Y.
AU - Zaabi, Mohammed Al
AU - Ali, Imran
N1 - Publisher Copyright:
© 2015 Taylor & Francis Group, LLC.
PY - 2015/8/9
Y1 - 2015/8/9
N2 - High-Performance Liquid Chromatography (HPLC) separation mechanisms of raspberry ketone and caffeine on phenyl columns (PhE and PPF) are described. The π-π interactions played major role in separation phenomenon. PhE column resulted into better separation than PPF due poor π-π interactions in later column. Fast, reproducible, selective, and effective HPLC method was developed for analyses of ketone and caffeine in pharmaceutical preparation. The columns used were Sunniest PhE (phenyl) (250 × 4.6 mm, 5.0 μm) and Sunshell PFP (100 × 4.6 mm, 2.6 μm) of Chromanik Japan. The mobile phase used was water-acetonitrile (60:40, v/v, at different pH values) at 1.0 mL/min flow rate. The detection was at 280 nm with 27 ± 1°C as working temperature. The ranges of capacity (k), separation (α), and resolution (Rs) factors at different pH values on both columns were 2.60-5.10, 1.32-1.60, and 3.22-4.28, respectively. The values of limit of detection (LOD) and limit of quantitation (LOQ) of raspberry ketone and caffeine on both columns ranged from 5.0 to 20.0 and 7.0 to 25.0, respectively. The developed mechanistic aspect is useful for designing separation strategies of other molecules on these phenyl columns. The reported method may be used for quality control in industries and government organizations. Besides, fast analyses may be carried out in various food products.
AB - High-Performance Liquid Chromatography (HPLC) separation mechanisms of raspberry ketone and caffeine on phenyl columns (PhE and PPF) are described. The π-π interactions played major role in separation phenomenon. PhE column resulted into better separation than PPF due poor π-π interactions in later column. Fast, reproducible, selective, and effective HPLC method was developed for analyses of ketone and caffeine in pharmaceutical preparation. The columns used were Sunniest PhE (phenyl) (250 × 4.6 mm, 5.0 μm) and Sunshell PFP (100 × 4.6 mm, 2.6 μm) of Chromanik Japan. The mobile phase used was water-acetonitrile (60:40, v/v, at different pH values) at 1.0 mL/min flow rate. The detection was at 280 nm with 27 ± 1°C as working temperature. The ranges of capacity (k), separation (α), and resolution (Rs) factors at different pH values on both columns were 2.60-5.10, 1.32-1.60, and 3.22-4.28, respectively. The values of limit of detection (LOD) and limit of quantitation (LOQ) of raspberry ketone and caffeine on both columns ranged from 5.0 to 20.0 and 7.0 to 25.0, respectively. The developed mechanistic aspect is useful for designing separation strategies of other molecules on these phenyl columns. The reported method may be used for quality control in industries and government organizations. Besides, fast analyses may be carried out in various food products.
KW - caffeine
KW - high-performance liquid chromatography analyses
KW - mechanisms of separation
KW - pharmaceutical extraction
KW - phenyland penta fluoro phenyl columns
KW - raspberry ketone
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U2 - 10.1080/10826076.2015.1048874
DO - 10.1080/10826076.2015.1048874
M3 - Article
AN - SCOPUS:84938833962
SN - 1082-6076
VL - 38
SP - 1324
EP - 1332
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
IS - 13
ER -