Local applications of GM-CSF induce the recruitment of immune cells in cervical low-grade squamous intraepithelial lesions

Pascale Hubert, Jean Doyen, Xavier Capelle, Mohammad Arafa, Virginie Renoux, Bettina Bisig, Laurence Seidel, Brigitte Evrard, Latifa Bousarghin, Colette Gerday, Jacques Boniver, Jean-Michel Foidart, Philippe Delvenne, Nathalie Jacobs

نتاج البحث: المساهمة في مجلةمراجعة النظراء

9 اقتباسات (Scopus)

ملخص

PROBLEM: Quantitative alterations of antigen-presenting cells (APC) in (pre)neoplastic lesions of the uterine cervix associated with human papillomavirus (HPV) infection suggest a diminished capacity to capture viral antigens and to induce a protective immune response.

METHOD OF STUDY: To test whether a cervical application of GM-CSF could restore an immune response against HPV in women with cervical low-grade squamous intraepithelial lesions (LSIL), we performed two clinical trials with 11 healthy women and 15 patients with LSIL.

RESULTS: GM-CSF applications were well tolerated in all enrolled women, and no difference in toxicity between the treated and placebo groups was observed during the follow-up (until 30 months). Interestingly, in the GM-CSF treated group, a significant increase of APC and cytotoxic T-lymphocyte infiltration was observed in the cervical biopsies with no change in regulatory T cell numbers. All the HPV16(+) patients exhibited an immune response against HPV16 after GM-CSF applications, as shown by NK and/or T cells producing IFN-gamma whereas no cellular immune response was observed before the treatment. Moreover, the anti-virus-like particles antibody titers also increased after the treatment.

CONCLUSION: These encouraging results obtained from a limited number of subjects justify further study on the therapeutic effect of APC in cervical (pre)neoplastic lesions.

اللغة الأصليةEnglish
الصفحات (من إلى)126-36
عدد الصفحات11
دوريةAmerican Journal of Reproductive Immunology and Microbiology
مستوى الصوت64
رقم الإصدار2
المعرِّفات الرقمية للأشياء
حالة النشرPublished - أغسطس 1 2010

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